Microbiomes of the Normal Middle Ear and Ears With Chronic Otitis Media
Microbiomes of the Normal Middle Ear and Ears With Chronic Otitis Media
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DOI：
10.1002/lary.26579
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发表时间：
2017-10-01
期刊：
影响因子：
2.6
通讯作者：
Kaga, Kimitaka
中科院分区：
文献类型：
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作者：
Minami, Shujiro B.;Mutai, Hideki;Kaga, Kimitaka
目的：研究慢性中耳炎患者和非慢性中耳炎患者中耳微生物区系的异同。研究设计：前瞻性多中心队列研究。方法：所有因慢性中耳炎接受鼓室成形术或非中耳炎患者接受耳部手术的患者均纳入研究对象。术中采集中耳粘膜无菌拭子样本。使用Illumina MiSeq测序仪(美国加利福尼亚州Illumina)的区域特异性引物扩增每个样品中16S rRNA基因的可变区4。对序列进行局部BLAST，并使用metagenome@kin(World Fusion，World Fusion，日本东京)进行分类。结果：总共从7个医学中心招募了155名参与者。其中88例为慢性中耳炎，67例为正常中耳。正常中耳粘膜表面最丰富的细菌门是变形杆菌，其次是放线杆菌、乳杆菌和拟杆菌。中耳正常的儿童和成人在中耳微生物群方面存在显著差异。无活动性炎症(干耳)的慢性中耳炎患者的中耳微生物群与正常中耳组相似。与正常中耳相比，慢性活动性中耳炎(湿耳)患者的变形杆菌感染率较低，而氟米米特的患病率较高。结论：人类中耳存在比先前认为的更多样化的微生物群落。中耳微生物群的改变可能参与了慢性中耳炎活动性炎症的发病机制。
Objective: The aim of this study was to profile and compare the middle ear microbiomes of human subjects with and without chronic otitis media.Study Design: Prospective multicenter cohort study.Methods: All consecutive patients undergoing tympanoplasty surgery for chronic otitis media or ear surgery for conditions other than otitis media were recruited. Sterile swab samples were collected from the middle ear mucosa during surgery. The variable region 4 of the 16S rRNA gene in each sample were amplified using region-specific primers adapted for the Illumina MiSeq sequencer (Illumina, CA, USA)). The sequences were subjected to local blast and classified using Metagenome@KIN (World Fusion, Tokyo, Japan).Results: In total, 155 participants were recruited from seven medical centers. Of these, 88 and 67 had chronic otitis media and normal middle ears, respectively. The most abundant bacterial phyla on the mucosal surfaces of the normal middle ears were Proteobacteria, followed by Actinobacteria, Firmicutes, and Bacteroidetes. The children and adults with normal middle ears differed significantly in terms of middle ear microbiomes. Subjects with chronic otitis media without active inflammation (dry ear) had similar middle ear microbiomes as the normal middle ears group. Subjects with chronic otitis media with active inflammation (wet ear) had a lower prevalence of Proteobacteria and a higher prevalence of Firmicutes than the normal middle ears.Conclusion: The human middle ear is inhabited by more diverse microbial communities than was previously thought. Alteration of the middle ear microbiome may contribute to the pathogenesis of chronic otitis media with active inflammation.