The crayfish plasma clotting protein:: A vitellogenin-related protein responsible for clot formation in crustacean blood
The crayfish plasma clotting protein:: A vitellogenin-related protein responsible for clot formation in crustacean blood
复制标题
DOI：
10.1073/pnas.96.5.1965
复制
发表时间：
1999-03-02
影响因子：
11.1
通讯作者：
Söderhäll, K
中科院分区：
文献类型：
--
作者：
Hall, M;Wang, RG;Söderhäll, K
小龙虾血液的凝固是基于转谷氨酰胺酶介导的特定血浆凝血蛋白的交联。在这里，我们报道了从小龙虾肝胰腺 cDNA 文库中克隆出的这种凝血蛋白的亚基。 ORF编码1,721个氨基酸的蛋白质，包括15个氨基酸的信号肽。序列分析表明，凝血蛋白与卵黄原蛋白同源，卵黄原蛋白是在产卵动物的卵黄发生雌性中发现的蛋白质。凝血蛋白和卵黄蛋白原都是脂蛋白，与某些其他脂蛋白（例如哺乳动物载脂蛋白 B 和微粒体甘油三酯转移蛋白）具有有限的序列相似性，并且包含与哺乳动物冯维勒布兰德因子的 D 结构域相似的一段序列。与雌性特有的卵黄原蛋白不同，小龙虾的凝血蛋白在两性中均存在。电子显微镜用于观察单个凝血蛋白分子并研究转谷氨酰胺酶介导的凝血反应。在内源性转谷氨酰胺酶存在的情况下，纯化的凝血蛋白分子迅速组装成长而柔韧的链，偶尔会分支。
Coagulation in crayfish blood is based on the transglutaminase-mediated crosslinking of a specific plasma clotting protein. Here we report the cloning of the subunit of this clotting protein from a crayfish hepatopancreas cDNA library. The ORF encodes a protein of 1,721 amino acids, including a signal peptide of 15 amino acids. Sequence analysis reveals that the clotting protein is homologous to vitellogenins, which are proteins found in vitellogenic females of egg-laying animals. The clotting protein and vitellogenins are all lipoproteins and share a limited sequence similarity to certain other lipoproteins (e.g., mammalian apolipoprotein B and microsomal triglyceride transfer protein) and contain a stretch with similarity to the D domain of mammalian von Willebrand factor. The crayfish clotting protein is present in both sexes, unlike the female-specific vitellogenins. Electron microscopy was used to visualize individual clotting protein molecules and to study the transglutaminase-mediated clotting reaction. In the presence of an endogenous transglutaminase, the purified clotting protein molecules rapidly assemble into long, flexible chains that occasionally branch.