DNA nanotechnology in the undergraduate laboratory: Electrophoretic analysis of DNA nanostructure biostability.
DNA nanotechnology in the undergraduate laboratory: Electrophoretic analysis of DNA nanostructure biostability.
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DNA纳米技术在本科生实验室：DNA纳米结构生物稳定性的电泳分析。
DOI：
10.1021/acs.jchemed.2c00656
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发表时间：
2023
影响因子：
3
通讯作者：
Chandrasekaran,ArunRichard
中科院分区：
文献类型：
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作者：
Chandrasekaran,ArunRichard
DNA纳米技术领域在过去十年中发展迅速，已经扩展到多个实验室。虽然一些机构已经开设了DNA纳米技术的课程，但是本科阶段的实验室仍然缺乏。本科生主要通过在研究实验室实习来学习DNA纳米技术。这里介绍的DNA纳米结构生物稳定性分析实验可以作为在本科环境中讨论DNA纳米技术概念的动手介绍性实验室练习。本实验探讨了一种模型DNA纳米结构——PX DNA基序的生物稳定性、凝胶电泳和核酸酶降解的定量分析。实验可以在化学、生物学或生物化学实验室进行，成本最低，也可以在本科院校使用这里提供的教师和学生手册进行调整。基于前沿研究的实验课程不仅为学生提供了一个直接的实践方法，而且可以增加本科生对研究的参与。此外，反映研究日益多学科性质的实验课程增加了本科教育的价值。
The field of DNA nanotechnology has grown rapidly in the past decade and has expanded to multiple laboratories. While lectures in DNA nanotechnology have been introduced in some institutions, laboratory components at the undergraduate level are still lacking. Undergraduate students predominantly learn about DNA nanotechnology through their involvement as interns in research laboratories. The DNA nanostructure biostability analysis experiment presented here can be used as a hands-on introductory laboratory exercise for discussing concepts in DNA nanotechnology in an undergraduate setting. This experiment discusses biostability, gel electrophoresis and quantitative analysis of nuclease degradation of a model DNA nanostructure, the paranemic crossover (PX) DNA motif. The experiment can be performed in a chemistry, biology, or biochemistry laboratory with minimal costs and can be adapted in undergraduate institutions using the instructor and student manuals provided here. Laboratory courses based on cutting edge research not only provide students a direct hands-on approach to the subject, but also can increase undergraduate student participation in research. Moreover, laboratory courses that reflect the increasingly multidisciplinary nature of research add value to undergraduate education.