Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried Cassava.
Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried Cassava.
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DOI：
10.3390/antiox5010006
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发表时间：
2016-02-02
期刊：
影响因子：
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通讯作者：
Isroli I
中科院分区：
文献类型：
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作者：
Sugiharto S;Yudiarti T;Isroli I
对从印尼发酵木薯中分离的两种丝状真菌（charticola Acremonium和oryzae Rhizopus）的抗氧化能力和抗氧化成分进行了研究。采用2,2′-氮基-双（3-乙基-苯并噻唑-6-磺酸）（ABTS）法对真菌粗提物的抗氧化能力进行了评价。采用Folin-Ciocalteu法测定总酚含量，采用氯化铝分光光度法测定总黄酮含量。用福林-丹尼斯法测定总单宁。与对照的稻瘟病菌和抗坏血酸相比，黄芪对ABTS+自由基的清除能力显著提高（p < 0.01）。黄芪总酚含量高于稻瘟病菌（p < 0.01）。稻瘟病菌的总黄酮含量显著高于黄芪（p < 0.01）。真菌a . charticola的总单宁含量高于r.m oryzae （p < 0.01）。综上所述，从印尼发酵木薯中分离的两种丝状真菌都具有清除ABTS+的能力，显示出抗氧化潜力。夏绿草的抗氧化能力高于稻瘟病菌。其抗氧化能力主要与酚类物质和单宁含量有关。
The antioxidant capacity and antioxidant constituents of two filamentous fungi (Acremonium charticola and Rhizopus oryzae) isolated from the Indonesian fermented dried cassava (gathot) were evaluated in the present study. The antioxidant capacity of the fungal crude extracts was assessed based on the 2,2′-azino-bis(3-ethyl-benzthiazolin-6-sulfonicacid) (ABTS) method. Total phenolics were determined based on the Folin-Ciocalteu method, while the flavonoids content in the fungal extracts was determined by the spectrophotometric method with aluminum chloride. Total tannins were estimated by the Folin-Denis method. The ABTS+ radical scavenging activity was higher (p < 0.01) in A. charticola compared to that in R. oryzae and ascorbic acid (as a control). A higher (p < 0.01) content of total phenolics was detected in A. charticola than that in R. oryzae. Total flavonoids were higher (p < 0.01) in R. oryzae as compared with that in A. charticola. The fungus A. charticola had a higher (p < 0.01) level of total tannins than R. oryzae. In conclusion, both filamentous fungi isolated from the Indonesian fermented dried cassava exhibited antioxidant potentials as indicated by their capabilities to scavenge ABTS+. A. charticola had a higher antioxidant capacity than R. oryzae. The antioxidant capacity of A. charticola was attributed mainly to its phenolics and tannins contents.