Effect of microtubules and intermediate filaments on mitochondrial distribution.
Effect of microtubules and intermediate filaments on mitochondrial distribution.
复制标题
微管和中间丝对线粒体分布的影响。
作者：
I. Summerhayes;D. Wong;L. B. Chen
激光染料罗丹明123特异性地染色活细胞中的线粒体，并便于在各种实验条件下观察单个细胞中线粒体分布的变化。可视化的线粒体在一些细胞系，然后处理这些细胞，研究不同的细胞骨架元素的间接免疫荧光，揭示了良好的，但不是绝对的相关性线粒体和微管或中间丝。同一细胞内的线粒体和微丝分布没有显示出这种相关性。根据各种实验方法的观察结果，我们认为线粒体分布受到微管和中间丝这两个系统的强烈影响。两者都没有发挥绝对的作用，但其中一个似乎能够在另一个缺席的情况下发挥更大的主导作用。
The laser dye rhodamine 123 specifically stains mitochondria in living cells and facilitates the observation of changes in mitochondrial distribution in single cells under a variety of experimental conditions. Visualization of mitochondria in a number of cell lines followed by processing of these cells to study different cytoskeletal elements by indirect immunofluorescence, revealed good but not absolute correlation between mitochondria and microtubules or intermediate filaments. Mitochondria and microfilament distribution within the same cell did not show such a correlation. On the basis of observations made by various experimental approaches, we suggest that mitochondrial distribution is under the strong influence of the two systems, microtubules and intermediate filaments. Neither plays an absolute role but one seems able to play a more dominant role in the absence of the other.