Induction of Hyphal Growth in Schizosaccharomyces japonicas.

Induction of Hyphal Growth in Schizosaccharomyces japonicas.
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日本裂殖酵母菌丝生长的诱导。

DOI:
10.1101/pdb.prot091868
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发表时间:
2016
期刊:
Cold Spring Harb Protoc
影响因子:
--
通讯作者:
H.
H.
中科院分区:
--
文献类型:
--
作者:
Niki;H.

文献摘要

相似文献

日本裂殖酵母是一种二相性酵母。根据营养条件的不同,酵母细胞生长和菌丝细胞生长之间的转变是可逆的。除了营养胁迫,诱导的DNA损伤,如喜树碱产生的损伤,也会诱导菌丝生长,而无论营养状态如何。本方案描述了两种诱导方法。
Schizosaccharomyces japonicus is a dimorphic yeast. Depending on the nutrient conditions, the transition between growth as yeast cells and growth as hyphal cells can be reversibly induced. In addition to nutrient stress, induced DNA lesions, such as those produced by camptothecin, also induce hyphal growth regardless of the nutrient status. This protocol describes both methods of induction.