Identification of quantitative trait loci for grain appearance using chromosome segment substitution lines in rice.
Identification of quantitative trait loci for grain appearance using chromosome segment substitution lines in rice.
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DOI：
10.1270/jsbbr.10.91
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发表时间：
2008-01-01
期刊：
影响因子：
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通讯作者：
Funane, M.
中科院分区：
文献类型：
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作者：
Ebitani, T.;Yamamoto, Y.;Funane, M.
稻米外观性状是水稻生产和育种中沿着产量和食味的重要性状之一。为了确定控制籽粒外观的染色体区域，我们进行了数量性状基因座（QTL）分析，使用染色体片段代换系（CSSLs）来自籼稻品种，Kasalath（供体）和粳稻品种，越光（经常性）之间的杂交。将CSSL的籽粒外观分为6类：完整籽粒、未成熟的白色籽粒，这些籽粒又分为白背籽粒、基部白色籽粒、白色浆果籽粒、乳白色籽粒和白色核心籽粒。在QTL定位中，以粒重比作为性状。结果表明，第1、2、5、6、7、8、9、10、11、12号染色体上的12个染色体区域参与了粒形性状的QTL定位。白背和基白籽粒的总百分比下降的Kasalath染色体区域的染色体2，9，11，和12的取代，但增加了5个区域的染色体2，5，6，8，和10。第1号染色体上的一个区域和第1、7号染色体上的两个区域的Kasalath等位基因分别增加了白色浆果和奶白色籽粒的比例。此外，我们进行了籽粒外观QTL定位，以验证染色体1，2，5，和12使用来自CSSLs和越光之间的杂交F2群体的QTL。结果，确认了4个QTL（RM3865或RM3703（chr2）、RM1208（chr12）、S1946（chr5）和RM8084（chr12））涉及引起CSSLs和越光之间的表型差异的基因。
Grain appearance is one of the most important traits along with yield and palatability in rice production and breeding programs. To identify the chromosomal regions controlling grain appearance, we performed quantitative trait locus (QTL) analysis using chromosome segment substitution lines (CSSLs) derived from a cross between an indica cultivar, Kasalath (donor), and a japonica cultivar, Koshihikari (recurrent). The grain appearances of CSSLs were classified into six categories: perfect grains, immature white grains subclassified as white-back kernels, basal-white kernels, white berry kernels, milk-white kernels, and white core kernels. The ratios of kernels were used as traits in QTL identification. It was suggested that 12 chromosomal regions of chromosomes 1 (two regions), 2 (two regions), 5, 6, 7, 8, 9, 10, 11, and 12 were involved in QTLs for grain appearance. The total percentage of white-back and basal-white kernels was decreased by the substitution of the Kasalath chromosomal regions of chromosomes 2, 9, 11, and 12, but was increased by five regions of chromosomes 2, 5, 6, 8, and 10. The Kasalath alleles in one region of chromosome 1 and two regions of chromosomes 1 and 7 increased the proportion of white berry and milk-white kernels individually. Furthermore, we performed QTL mapping for grain appearance to validate the QTLs of chromosomes 1, 2, 5, and 12 using four F2 populations derived from a cross between CSSLs and Koshihikari. As a result, it was confirmed that four QTLs (RM3865 or RM3703 (chr2), RM1208 (chr12), S1946 (chr5), and RM8084 (chr12)) were involved in the gene causing phenotypic differences between the CSSLs and Koshihikari.