Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering
Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering
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DOI：
10.1016/j.biomaterials.2004.07.026
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Ramakrishna, S
中科院分区：
文献类型：
--
作者：
Ma, ZW;Kotaki, M;Ramakrishna, S
采用静电纺丝技术制备了非织造聚对苯二甲酸乙二酯纤维毡（PET NFM），并对其进行表面改性，以模拟天然细胞外基质中的纤维蛋白，构建内皮细胞生物相容性表面。静电纺丝PET NFM首先在甲醛中处理以在表面上产生羟基，然后在Ce（IV）引发下接枝甲基丙烯酸（MAA）。最后，以水溶性碳二亚胺为偶联剂，将PMMA接枝PET NFM与明胶接枝。对平面PET薄膜进行了表面改性和表征，对表面改性过程有了基本的了解。用XPS和比色法对PET表面接枝PMAA和明胶进行了定量分析。将内皮细胞分别接种于原代和明胶修饰的PET NFM上，观察细胞形态、增殖和活力。用免疫荧光显微镜研究了内皮细胞表达的三种特征性表面标志物。明胶接枝法能明显促进内皮细胞在PET核基质上的铺展和增殖，并能保持内皮细胞的表型。(C)2004 Elsevier Ltd.保留所有权利。
Non-woven polyethylene terephthalate nanofiber mats (PET NFM) were prepared by electrospinning technology and were surface modified to mimic the fibrous proteins in native extracellular matrix towards constructing a biocompatible surface for endothelial cells (ECs). The electrospun PET NFM was first treated in formaldehyde to yield hydroxyl groups on the surface, followed by the grafting polymerization of methacrylic acid (MAA) initiated by Ce(IV). Finally, the PMAA-grafted PET NFM was grafted with gelatin using water-soluble carbodiimide as coupling agent. Plane PET film was also surface modified and characterized for basic understanding of the surface modification process. The grafting of PMAA and gelatin on PET surface was confirmed by XPS spectroscopy and quantitatively analyzed by colorimetric methods. ECs were cultured on the original and gelatin-modified PET NFM and the cell morphology, proliferation and viability were studied. Three characteristic surface makers expressed by ECs were studied using immuno-florescent microscopy. The gelatin grafting method can obviously improve the spreading and proliferation of the ECs on the PET NFM, and moreover, can preserve the EC's phenotype. (C) 2004 Elsevier Ltd. All rights reserved.