STEROID-SECRETION BY CUMULUS CELLS ISOLATED FROM HUMAN PREOVULATORY FOLLICLES
STEROID-SECRETION BY CUMULUS CELLS ISOLATED FROM HUMAN PREOVULATORY FOLLICLES
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DOI：
10.1530/acta.0.1080407
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
ANDINO, N
中科院分区：
文献类型：
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作者：
HILLENSJO, T;SJOGREN, A;ANDINO, N
在体外培养6-24 h的人卵母细胞-卵丘复合体中检测孕酮、睾酮和雌二醇的分泌。这些复合物是从32名妇女的排卵前卵泡中吸出的，这些妇女由于输卵管疾病而参加了体外受精计划。在12名妇女的卵泡成熟诱导克罗米酚和人绒毛膜促性腺激素（hCG），在13名妇女与人绝经期促性腺激素（hMG）和hCG和7名妇女与克罗米酚-hMG加hCG的组合。通过在特定时间取出等分试样的放射免疫测定法，研究了添加精子前（0-6 h）和添加精子后（6-24 h）受精培养基中类固醇的净分泌。在授精前后均能持续分泌大量孕酮。睾酮分泌保持在一个低的和恒定的水平，而净释放的雌二醇主要是在第1小时的孵化。类固醇，特别是孕酮的释放，根据体内激素刺激的模式而变化，并且在克罗米酚-hMG治疗的妇女的复合物中最高，这可能反映了抽吸卵泡的不同成熟度。在第二系列实验中，在受精后回收分散的卵丘细胞，并将其作为单层培养2-4天。细胞自发黄体化并分泌大量孕酮。这些结果扩展了以前在动物中的工作，表明在人类中，排卵期卵丘细胞也具有类固醇生成活性。卵丘细胞的功能差异与诱导排卵的方式有关。
The secretion of progesterone, testosterone and estradiol by intact human occyte-cumulus complexes in vitro was examined in incubations lasting 6-24 h. The complexes were aspirated from preovulatory follicles in 32 women who, due to tubal disease, were participating in an in vitro fertilization program. In 12 of the women follicular maturation was induced with clomiphene and human chorionic gonadotropin (hCG), in 13 women with human menopausal gonadotropin (hMG) and hCG and in 7 women with a combination of clomiphene-hMG plus hCG. The net secretion of steroids into the fertilization medium was studied before (0-6 h) and after (6-24 h) the addition of sperm, by radioimmunoassay of aliquots removed at specific times. A high and sustained secretion of progesterone was found both before and after insemination. Testosterone secretion remained at a low and constant level while a net relase of estradiol was found mainly during the 1st h of incubation. The release of steroids, particularly progesterone, varied according to the mode of hormonal stimulation in vivo and was highest in complexes from clomiphene-hMG-treated women, probably reflecting different maturity of the aspirated follicles. In a 2nd series of experiments the dispersed cumulus cells were recovered after fertilization and cultured as monolayers for 2-4 days. The cells underwent spontaneous luteinization and secreted high amounts of progesterone. These results extend previous work in animals showing that also in the human the periovulatory cumulus cells are steroidogenically active. A functional difference in the cumulus cells related to the mode of ovulation induction was suggested.