Cu induced changes of ultrastructure and bioaccumulation in the leaf of Moso bamboo (Phyllostachys pubescens)
Cu induced changes of ultrastructure and bioaccumulation in the leaf of Moso bamboo (Phyllostachys pubescens)
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DOI：
10.1080/01904167.2017.1380816
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发表时间：
2018-01
影响因子：
2.1
通讯作者：
Song Li;Ying Wang;Q. Mahmood;Jun-ren Chen;Jia-sen Wu;Zhengqian Ye;D. Peng;W. Yan;Kou-ping Lu-Kou-pi
中科院分区：
文献类型：
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作者：
Song Li;Ying Wang;Q. Mahmood;Jun-ren Chen;Jia-sen Wu;Zhengqian Ye;D. Peng;W. Yan;Kou-ping Lu-Kou-pi
摘要 研究了铜 (Cu) 对毛竹 (Phyllostachys hetrocycla (Carr.) Mitford cv. pubescens) 生长的影响。铜胁迫下毛竹的干重受到抑制，与对照（CK）相比，差异显着。溶液培养实验表明，毛竹在地上部积累Cu的能力很强。茎中铜的积累强于叶片； Cu胁迫下茎根比可达0.20。通过扫描电镜和透射电镜观察细胞壁、气孔、液泡和淀粉粒含量的变化。超微结构分析表明，过量的铜浓度导致叶绿体畸形和内质网转化甚至消失。毛竹作为实验材料，由于其金属耐受性和过量的生物质产量，成为修复重金属污染土壤的新工具。
ABSTRACT The effects of Copper (Cu) on growth of Moso bamboo (Phyllostachys heterocycla (Carr.) Mitford cv. pubescens) were investigated. The dry weight of Moso bamboo was inhibited and significant differences were evident under Cu stress as compared to the control (CK). Solution culture experiments indicated that Moso bamboo had a strong ability to accumulate Cu in shoots. Copper accumulation in the stem was stronger than the leaves; the stem/root ratio could reach 0.20 under Cu stress. The changes in cell walls, stomata, vacuoles, and starch grains content were observed via scanning electron microscopy and transmission electron microscopy. The ultrastructural analysis revealed that the excessive Cu concentrations caused deformities in the chloroplasts and transformation and even disappearance of endoplasmic reticulum. Moso bamboo as an experimental material serves as a new tool for remediation of heavy metal contaminated soils due to its metal tolerance and excessive biomass production.