Expression and distribution of aquaporin 3 in human placenta and fetal membranes
Expression and distribution of aquaporin 3 in human placenta and fetal membranes
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DOI：
10.3760/cma.j.issn.1674-1927.2007.04.005
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发表时间：
2007-08
期刊：
影响因子：
3.8
通讯作者：
Hui-shu Liu;Wenjing Yuan
中科院分区：
文献类型：
--
作者：
Hui-shu Liu;Wenjing Yuan
目的 探讨水通道蛋白3（AQP3）在正常人胎盘和胎膜中的表达和分布。方法 采集5例足月剖宫产胎膜完整的正常孕妇的胎盘和胎膜，采用逆转录聚合酶链式反应（RT-PCR）、Western blot分析和免疫组化检测AQP3的表达和定位。胎膜和胎膜Western blot检测也呈阳性，在29 000左右有特异条带位点。免疫组化定位显示AQP3存在于胎盘合体滋养层，而羊膜上皮细胞无信号染色。结论AQP3可能在母胎体液平衡中发挥重要作用。关键词: 水通道蛋白3;胎盘;胎膜；体液；基因表达
Objective To investigate the expression and distribution of aquaporin 3(AQP3)in normal human placenta and fetal membranes.Methods Placenta and fetal membrane were collected from 5 normal women with intact membranes undergone elective cesarean sections at term.Expression and localization of AQP3 was detected by reverse transcriptase-polymerase chain reaction(RT-PCR),Western blot analysis and immunohistochemistry.Results AQP3 messenger RNA was found in placenta and fetal membrane.Western blot analysis also yielded positive results for placenta and fetal membrane with specific band site around 29 000.Immunohistochemical localization showed AQP3 Was present at syncytotrophoblast of placenta and there was no signal staining at epithelial cell of amnion.Condusion AQP3 may play an important role in maternal-fetal fluid balance. Key words: Aquaporin 3; Placenta; Fetal membranes; Body fluids; Gene expression