Physical, chemical, and physiological characteristics of isolates of pulmonary surfactant from adult rabbits.
Physical, chemical, and physiological characteristics of isolates of pulmonary surfactant from adult rabbits.
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成年兔肺表面活性物质分离物的物理、化学和生理特性。
DOI：
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发表时间：
1985
影响因子：
2.1
通讯作者：
H. Taeusch
中科院分区：
文献类型：
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作者：
K. Keough;E. Farrell;M. Cox;G. Harrell;H. Taeusch
测定了两种成年兔表面活性物质制剂的理化性质。使用多次离心机的过程产生了一种表面活性物质(A型)，其具有6.6%的重量蛋白质和13.1nmolP/微克蛋白质的磷/蛋白质比。一种更简单的方法，包括两次离心法，产生了一种表面活性物质(B型)，含有更多的蛋白质(10.8%)和较低的磷/蛋白质比(8.4nmol/微克)。两种类型的脂肪组成相似，磷脂酰胆碱是主要的磷脂(80%)，棕榈酸酯是总脂中的主要脂肪酸(65-71%)，磷脂酰胆碱(80%)是主要的脂肪酸。这两种类型都表现出宽的热致相变，包括37℃。在朗缪尔-威尔赫米天平表面或在脉动气泡装置中对表面活性物质水分散体的测量表明，在表面平衡上达到低表面张力的能力和在气泡装置上实现低表面张力的速率方面，在类型之间和在相同类型的批次之间都存在差异。在满足这些目的方面，A型制剂在某种程度上比B型制剂更可靠。两种类型的分离株在注入未成熟兔胎儿的肺中时，在使压力-体积特征正常化方面都是有效的。
Physical and chemical characteristics of two types of preparations of surface active material from adult rabbits were determined. A procedure using multiple centrifugations produced a surface active material (type A) which had 6.6% by weight protein and a phosphorus/protein ratio of 13.1 nmol P/microgram protein. A simpler protocol involving two centrifugations yielded a surface active material (type B) with more protein (10.8%) and a lower phosphorus/protein ratio (8.4 nmol/micrograms). Lipid compositions of both types were similar with phosphatidylcholine being the major phospholipid (80%) and palmitate the major fatty acid in the total lipid (65-71%) and in phosphatidylcholine (80%). Both types exhibited broad thermotropic phase transitions encompassing 37 degrees C. Measurements of aqueous dispersions of surface active material on the surface of a Langmuir-Whilhelmy balance or in a pulsating bubble apparatus indicated that there was variability both between types and between batches of the same type in the capacity to reach low surface tension on the surface balance and in the rates at which low surface tension was achieved on the bubble apparatus. Type A preparations were somewhat more reliable in meeting these ends than were type B. Both types of isolates were effective in normalizing pressure-volume characteristics when instilled into the lungs of immature rabbit fetuses.