Genomic resources and draft assemblies of the human and porcine varieties of scabies mites, Sarcoptes scabiei var. hominis and var. suis
Genomic resources and draft assemblies of the human and porcine varieties of scabies mites, Sarcoptes scabiei var. hominis and var. suis
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DOI：
10.1186/s13742-016-0129-2
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发表时间：
2016-06-02
期刊：
影响因子：
9.2
通讯作者：
Papenfuss, Anthony T.
中科院分区：
文献类型：
--
作者：
Mofiz, Ehtesham;Holt, Deborah C.;Papenfuss, Anthony T.
背景资料：疥螨，Sarcoptes scabiei，是一种寄生性蜘蛛纲动物，是人类传染性皮肤病疥疮和其他动物疥螨的病因。疥疮感染是一个主要的健康问题，特别是在偏远的土著社区在澳大利亚，在那里的第二组链球菌和金黄色葡萄球菌感染疥疮疮被认为是驱动风湿性心脏病和慢性肾脏病的高发病率。使用Illumina HiSeq从位于澳大利亚北方不同地区的患有结痂疥疮的无关患者获得的人。我们还测序样品疥螨变种。猪的模型。由于疥螨的体积很小，这些数据来自数千只螨的库，并且是宏基因组，包括宿主和微生物组DNA。我们进行了清洁和从头组装，并提出疥螨变种。hominis和var. suis草案参考基因组。我们已经构建了一个初步的注释，这个参考包括13,226推定的编码序列的基础上的序列相似性已知protein.Conclusions：我们已经开发了广泛的基因组资源疥螨，包括参考基因组和初步注释。
Background: The scabies mite, Sarcoptes scabiei, is a parasitic arachnid and cause of the infectious skin disease scabies in humans and mange in other animal species. Scabies infections are a major health problem, particularly in remote Indigenous communities in Australia, where secondary group A streptococcal and Staphylococcus aureus infections of scabies sores are thought to drive the high rate of rheumatic heart disease and chronic kidney disease.Results: We sequenced the genome of two samples of Sarcoptes scabiei var. hominis obtained from unrelated patients with crusted scabies located in different parts of northern Australia using the Illumina HiSeq. We also sequenced samples of Sarcoptes scabiei var. suis from a pig model. Because of the small size of the scabies mite, these data are derived from pools of thousands of mites and are metagenomic, including host and microbiome DNA. We performed cleaning and de novo assembly and present Sarcoptes scabiei var. hominis and var. suis draft reference genomes. We have constructed a preliminary annotation of this reference comprising 13,226 putative coding sequences based on sequence similarity to known proteins.Conclusions: We have developed extensive genomic resources for the scabies mite, including reference genomes and a preliminary annotation.