A NEW CLUSTER OF GENES WITHIN THE HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX
A NEW CLUSTER OF GENES WITHIN THE HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX
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DOI：
10.1126/science.2911734
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发表时间：
1989-01-13
期刊：
影响因子：
56.9
通讯作者：
STROMINGER, JL
中科院分区：
文献类型：
--
作者：
SPIES, T;BLANCK, G;STROMINGER, JL
用重叠互补链染色体步移法分离到一个435-kb的DNA片段，它位于人类主要组织相容性复合体中HLA-B的着丝粒区。在克隆的区域内，肿瘤坏死因子（TNF）α的基因。和β与HLA-B的同源性为210 kb。小鼠基因B144的人同源物位于TNF α附近。此外，大量的CpG岛表明存在其他基因。粘粒探针，几个不同的成绩单中检测到的RNA样品从各种细胞系。通过分离相应的互补DNA克隆，共鉴定出5个新基因。这些“HLA-B.sbd.相关转录本”（BAT）被定位到包括TNF α基因的160-kb区域内的不同位置。和TNF β。在HLA-B附近存在的BAT 1至BAT 5基因再次提出了一个问题，即该区域的哪个基因决定了强直性脊柱炎的易感性。
A 435-kilobase (kb) DNA segment, which is centromeric to HLA-B in the human major histocompatibility complex, was isolated by chromosome walking with overlapping cosmids. Within the cloned region, the genes for the tumor necrosis factors (TNFs) .alpha. and .beta. and HLA-B were 210 kb apart. The human homolog of a mouse gene, B144, was located next to TNF.alpha.. Moreover, the presence of additional genes was suggested by a large cluster of CpG islands. With cosmid probes, several distinct transcripts were detected in RNA samples from a variety of cell lines. Altogether, five novel genes were identified by isolation of corresponding complementary DNA clones. These "HLA-B.sbd.associated transcripts" (BATs) were mapped to different locations within a 160-kb region that includes the genes for TNF.alpha. and TNF.beta.. The presence of the genes for BAT1 to BAT5 in the vicinity of HLA-B again raises the question of which gene in this region determines susceptibility to ankylosing spondylitis.