Intra-articular delivery of umbilical cord-derived mesenchymal stem cells temporarily retard the progression of osteoarthritis in a rat model
Intra-articular delivery of umbilical cord-derived mesenchymal stem cells temporarily retard the progression of osteoarthritis in a rat model
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关节内输送脐带间充质干细胞可暂时延缓大鼠模型骨关节炎的进展
DOI：
10.1111/1756-185x.13834
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发表时间：
2020-04-21
影响因子：
2.5
通讯作者：
Lin, Jianhao
中科院分区：
文献类型：
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作者：
Xing, Dan;Wu, Jun;Lin, Jianhao
目的探讨以间充质干细胞（MSC）为基础的骨关节炎（OA）治疗方法。人脐带间充质干细胞（Human umbilical cord-derived mesenchymal stem cells，hUC-MSCs）的研究报道较少。在这项研究中，我们研究了单关节内注射hUC-MSCs对大鼠OA模型的影响。方法从人脐带的沃顿氏胶中分离hUC-MSCs并鉴定。18只Sprague-Dawley大鼠用于OA模型。将大鼠分为3组：透明质酸（HA）+MSCs组（n = 6）、HA组（n = 6）和对照组（n = 6）。将在100 μ L HA、100 μ L HA或100 μ L盐水中的1 × 10（6）个hUC-MSC作为单剂量在手术后4周注射到膝关节中。在治疗后6周和12周，通过宏观检查、显微计算机断层扫描分析、行为分析和组织学检查对软骨退变进行评估。结果在6周时，HA + MSCs组的股骨髁评分明显优于HA组和对照组。组织学分析还显示HA + MSC组中蛋白多糖更多，软骨损失更少，改良Mankin评分更低。然而，在12周时，从肉眼检查和组织学分析来看，组间无显著差异。软骨下骨硬化的股骨内侧髁和行为测试显示组间在6和12 wk.Conclusion这些研究结果表明，单次注射hUC-MSCs可以有暂时的效果，减缓OA大鼠软骨退变的进展，但可能不会抑制OA的进展，在长期。
Aim Mesenchymal stem cell (MSC)-based therapy is being explored in treating osteoarthritis (OA). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are least reported. In this study, we investigated the effects of single intra-articular injections of hUC-MSCs on a rat OA model.Method hUC-MSCs were isolated from the Wharton's jelly of the human umbilical cord and identified. Eighteen Sprague-Dawley rats were used for the OA model. All rats were divided into 3 groups: hyaluronic acid (HA)+MSCs (n = 6), HA (n = 6), and control group (n = 6). One by 10(6) hUC-MSCs in 100 mu L HA, 100 mu L HA or 100 mu L saline were injected into the knee joint 4 weeks post-surgery as a single dose. Cartilage degeneration was evaluated at 6 and 12 weeks after treatment with macroscopic examination, micro-computed tomography analysis, behavioral analysis, and histology.Results At 6 weeks, the HA + MSCs group had a significantly better International Cartilage Repair Society score in the femoral condyle compared to the HA and control groups. Histological analysis also showed more proteoglycan and less cartilage loss, with lower modified Mankin score in the HA + MSCs group. However, at 12 weeks there were no significant differences between groups from macroscopic examination and histological analysis. Subchondral bone sclerosis of the medial femoral condyle and behavioral tests showed no significant differences between groups at 6 and 12 weeks.Conclusion These findings indicate that single injection of hUC-MSCs can have temporary effects on decelerating the progression of cartilage degeneration in OA rats, but may not inhibit OA progression in the long-term.