1,25-Dihydroxycholecalciferol: A Potent Stimulator of Bone Resorption in Tissue Culture
1,25-Dihydroxycholecalciferol: A Potent Stimulator of Bone Resorption in Tissue Culture
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1,25-二羟基胆钙化醇：组织培养中骨吸收的有效刺激剂
作者：
L. Raisz;C. Trummel;M. Holick;H. DeLuca
从与25-羟基胆钙化醇（HCC）孵育的肾匀浆中分离的1，25-二羟基胆钙化醇（DHCC）在10-10至10- 8 M的浓度下刺激了先前掺入的4545 Ca从器官培养的胎鼠骨中的释放。1，25-DHCC和25-HCC（母体化合物）的剂量反应曲线是平行的，但1，25-DHCC在重量基础上的效力约为100倍。短暂暴露于最大剂量的任一药剂导致骨吸收延长。
1,25-Dihydroxycholecalciferol (DHCC), isolated from kidney homogenates incubated with 25-hydroxycholecalciferol (HCC), stimulated the release of previously incorporated 4545Ca from fetal rat bones in organ culture, at concentrations of 10-10 to 10-8M. The dose response curves for 1,25-DHCC and 25-HCC, the parent compound, are parallel, but 1,25-DHCC is about 100 times as potent on a weight basis. Brief exposure to maximum doses of either agent leads to prolonged bone resorption.