A primary tumor gene expression signature identifies a crucial role played by tumor stroma myofibroblasts in lymph node involvement in oral squamous cell carcinoma.
A primary tumor gene expression signature identifies a crucial role played by tumor stroma myofibroblasts in lymph node involvement in oral squamous cell carcinoma.
复制标题
DOI：
10.18632/oncotarget.20645
复制
发表时间：
2017-12-01
期刊：
影响因子：
--
通讯作者：
Lo Muzio L
中科院分区：
文献类型：
--
作者：
Mazzoccoli G;Castellana S;Carella M;Palumbo O;Tiberio C;Fusilli C;Capocefalo D;Biagini T;Mazza T;Lo Muzio L
口腔鳞状细胞癌（OSCC）是最常见的口腔和咽癌，在西方世界约占男性癌症的3%和女性癌症的2%，发展中国家的发病率不断上升。早期发现的筛查是必要的，以防止致命的疾病，因为早期，可治愈的病变很少有症状。当手术、放疗或两者都作为治疗选择时，总体5年生存率约为50%，但淋巴结受累极大地影响了这一估计，使生存率降低了约50%。在这里，我们的目的是寻找与OSCC患者淋巴结转移相关的遗传特征。我们通过微阵列表达谱对一组无淋巴结受累患者（10例，平均年龄±SD 61.2±13.8，男性7例，女性3例）和有淋巴结受累患者（11例，平均年龄±SD 62.1±15.1，男性8例，女性3例）的OSSC标本进行全转录组分析，解决了这一问题。我们在淋巴结受累的OSCC患者标本中证实了与肌肉收缩相关基因的基因表达特征。这一基因标记表明，淋巴结受损伤患者的肿瘤造口中存在肌成纤维细胞，并强调了肌成纤维细胞可能通过分泌组决定OSCC侵袭性的决定性作用。
Oral squamous cell carcinoma (OSCC) is the most common oral and pharyngeal cancer, and is responsible of approximately 3% of cancers in men and 2% in women in the Western World, with increasing incidence rates in developing countries. Early detection by screening is necessary to prevent fatal disease because early, curable lesions are rarely symptomatic. The overall 5-yr survival rate is approximately 50% when surgery, radiation, or both are employed as treatment options, but lymph node involvement greatly influences this estimate, by decreasing the survival rate by about 50%. Here, we aimed at finding genetic signatures associated with lymph node metastasis in OSCC patients. We addressed this issue by whole transcriptome analysis through microarray expression profiling of a set of OSSC specimens of patients without lymph node involvement (10 patients, mean age ± SD 61.2±13.8, male 7, female 3) and with lymph node involvement (11 patients, mean age ± SD 62.1±15.1, male 8, female 3). We evidenced a gene expression signature associated to muscle contraction-related genes in specimens obtained from OSCC patients with lymph node involvement. This gene signature suggests the presence of myofibroblasts in tumor stoma of patients with lymph node involvement and emphasizes the decisive role played by myofibroblasts probably through their secretome in determining OSCC invasiveness.