Isolation and characterization of rhizosphere bacteria with potential for biological control of weeds in vineyards
Isolation and characterization of rhizosphere bacteria with potential for biological control of weeds in vineyards
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DOI：
10.1111/j.1365-2672.2006.02851.x
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发表时间：
2006-05-01
影响因子：
4
通讯作者：
O'Hara, G. W.
中科院分区：
文献类型：
--
作者：
Flores-Vargas, R. D.;O'Hara, G. W.
目的：有害根际抑制细菌（DRB）具有抑制植物生长的潜力。该项目的重点是隔离DRB与潜在的开发作为商业产品的杂草control.Methods和结果：细菌被分离出根际，根面，和根际的幼苗和成熟植物的野生萝卜（萝卜），一年生黑麦草（硬实黑麦草）和山羊草（Arctotheca austula）生长在葡萄园中的天鹅谷，西澳大利亚州。大多数（81.5%）的442菌株获得的根际或根面。快速筛选技术的发展，以评估在实验室和温室细菌对植物的影响。在温室中筛选菌株对一年生黑麦草、野萝卜、葡萄藤根（葡萄属葡萄）和豆科覆盖作物地下三叶草（三叶草）的有害影响。经Biolog系统和16S rRNA基因测序鉴定，3株菌分别为荧光假单胞菌（WSM3455和WSM3456）和木糖氧化产碱菌（WSM3457）。其中一株荧光假单胞菌（WSM 3455）产生氰化氢，氰化氢是一种植物根系的抑制剂，也是一种广谱抗菌化合物。结论：三株菌株特异性抑制野萝卜，但对葡萄藤根或地下三叶草没有显著的有害影响。本研究表明，操纵杂草幼苗根际鉴定DRB作为一个潜在的生物控制剂，野萝卜。
Aims: Deleterious rhizosphere inhabiting bacteria (DRB) have potential to suppress plant growth. This project focuses on the isolation of DRB with potential for development as commercial products for weed control.Methods and Results: Bacteria were isolated from the rhizosphere, rhizoplane, and endorhizosphere of seedlings and mature plants of wild radish (Raphanus raphanistrum), annual ryegrass (Lolium rigidum) and capeweed (Arctotheca calendula) growing in vineyards in the Swan Valley, Western Australia. A majority (81.5%) of the 442 strains was obtained from either rhizospheres or rhizoplanes. Rapid screening techniques were developed to evaluate in the laboratory and glasshouse the effects of bacteria on plants. Strains were screened in the glasshouse for deleterious effects on annual ryegrass, wild radish, grapevine rootlings (Vitis vinifera) and the legume cover crop subterranean clover (Trifolium subterraneum). Three strains were identified using the Biolog system and 16S rRNA gene sequencing as two strains of Pseudomonas fluorescens (WSM3455 and WSM3456) and one strain of Alcaligenes xylosoxidans (WSM3457). One of the P. fluorescens (WSM3455) strain produced hydrogen cyanide, an inhibitor of plant roots and a broad-spectrum antimicrobial compound.Conclusions: Three strains specifically inhibited wild radish but had no significant deleterious effects on either grapevine rootlings or subterranean clover.Significance and Impact of the Study: This study suggested manipulation of the weed seedling rhizosphere using identified DRB as a potential biocontrol agent for wild radish.