Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China
Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China
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中国猪流行性腹泻病毒GII亚型变异株的分离和鉴定
DOI：
10.1016/j.micpath.2019.103922
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发表时间：
2020-03-01
影响因子：
3.8
通讯作者：
Yin, Baishuang
中科院分区：
文献类型：
--
作者：
Yang, Dan;Su, Mingjun;Yin, Baishuang
背景资料：方法：分离纯化猪流行性腹泻病毒（PEDV）变异株HM 2017，根据组织培养半数感染剂量（TCID 50）建立病毒生长曲线。用RT-PCR方法扩增HM 2017基因，并进行遗传学分析。结果：基于基因组的系统发育分析表明，PEDV HM 2017株与目前在我国猪群中流行的变异株GII-a聚为一个亚群。在Vero细胞中传代15次后，菌株HM 2017的最高中位组织培养感染剂量为1.33 x 10（7）个病毒颗粒/mL。菌株HM 2017对哺乳仔猪具有高毒性，其在感染后12小时（hpi）表现出临床症状（即，结论：本研究报道了我国一株变异亚群GII-a PEDV HM 2017株，并对其致病性进行了初步鉴定。GII-a亚群PEDV株HM 2017为控制中国PED暴发提供了有希望的疫苗候选物。
Background: Highly virulent variants of porcine epidemic diarrhea virus (PEDV) have been closely associated with recent outbreaks of porcine epidemic diarrhea (PED) in China, which have resulted in severe economic losses to the pork industry.Methods: In the current study, the variant PEDV strain HM2017 was isolated and purified and a viral growth curve was constructed according to the median tissue culture infective dose (TCID50). HM2017 were amplify with RT-PCR and analyzed by phylogeny analysis. Animal pathogenicity experiment was carried to evaluate the HM2017 clinical assessment.Results: Genome-based phylogenetic analysis revealed that PEDV strain HM2017 was clustered into the variant subgroup GII-a that is currently circulating in pig populations in China. The highest median tissue culture infectious dose of strain HM2017 after 15 passages in Vero cells was 1.33 x 10(7) viral particles/mL. Strain HM2017 was highly virulent to suckling piglets, which exhibited clinical symptoms at 12 h post-infection (hpi) (i.e., weight loss at 12-84 hpi, increased body temperatures at 24-48 hpi, high viral loads in the jejunum and ileum, and 100% mortality by 84 hpi).Conclusion: The present study reports a variant subgroup GII-a PEDV HM2017 strain in China and characterize its pathogenicity. PEDV strain HM2017 of subgroup GII-a presents a promising vaccine candidate for the control of PED outbreaks in China.