Dynamics of B-DNA in the solid state.
Dynamics of B-DNA in the solid state.
复制标题
固态 B-DNA 的动力学。
DOI：
10.1016/0022-2836(81)90304-1
复制
发表时间：
1981
影响因子：
5.6
通讯作者：
Opella,SJ
中科院分区：
文献类型：
--
作者：
DiVerdi,JA;Opella,SJ
水合固体b型DNA在5°C以上的温度下具有磷酸二酯基团和固定碱基的大振幅运动。与104Hz相比，固态DNA的31p核磁共振化学位移粉末模式通过快速运动显着平均。DNA通过交换在C-8嘌呤位置用氘标记。2h核磁共振波谱具有完整的静态四极杆粉末模式，表明与50°C下106Hz相比，没有快速运动。
Hydrated solid B-form DNA is shown to have large amplitude motions of the phosphodiester groups and immobile bases at temperatures above 5 °C. The31P nuclear magnetic resonance chemical shift powder pattern of DNA in the solid state is significantly averaged by motions that are rapid compared to 104Hz. The DNA was labelled with deuterium at the C-8 purine position by exchange. The2H nuclear magnetic resonance spectra have complete static quadrupole powder patterns indicative of no motions fast compared to 106Hz at 50 °C.