Aldehyde dehydrogenase is a positional marker in the retina.
Aldehyde dehydrogenase is a positional marker in the retina.
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DOI：
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发表时间：
1991-07
期刊：
影响因子：
4.6
通讯作者：
P. McCaffery;P. Tempst;G. Lara;U. C. Dräger
中科院分区：
文献类型：
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作者：
P. McCaffery;P. Tempst;G. Lara;U. C. Dräger
通过蛋白质显微测序鉴定，胚胎小鼠视网膜中存在一种不对称分布的蛋白质，即乙醛脱氢酶。它的特征是具有基本等电点和偏爱脂肪底物的胞液异构体，其特征类似于已知的将视黄醛氧化为维甲酸的异构体AHD-2。乙醛脱氢酶抗血清免疫组织化学染色显示，从早期眼泡到成年期，视网膜背侧有很强的标记。此外，起源于视网膜背侧的视轴突在其生长阶段被瞬时标记。而在胚胎中，该酶在未分化的细胞和神经元中表达，而在成年小鼠的视网膜中，不对称分布的异构体主要在Müler胶质细胞中表达，标记的胶质细胞的数量随着视网膜位置的不同而不同。
An asymmetrically distributed protein in the embryonic mouse retina was identified as an aldehyde dehydrogenase through protein microsequencing. It was characterized as a cytosolic isoform with basic isoelectric point and preference for aliphatic substrates, features that resemble those of the isoform AHD-2 which is known to oxidize retinaldehyde to retinoic acid. Immunohistochemistry with aldehyde dehydrogenase antisera showed strong labeling of the dorsal retina from the early eye vesicle stage into adulthood. In addition, optic axons originating from the dorsal retina were transiently labeled during their outgrowth phase. Whereas in the embryo the enzyme was expressed in undifferentiated cells and in neurons, in the retina of the adult mouse the asymmetrically distributed isoform was mainly expressed in Müller glia, with the number of labeled glial cells varying with retinal position.