Action of bacterial neutral protease on the dispersion of mammalian cells in tissue culture.
Action of bacterial neutral protease on the dispersion of mammalian cells in tissue culture.
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细菌中性蛋白酶对组织培养中哺乳动物细胞分散的作用。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
H. Katsuta
中科院分区：
文献类型：
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作者：
T. Matsumura;K. Nitta;M. Yoshikawa;T. Takaoka;H. Katsuta
研究了细菌中性蛋白酶对培养细胞的作用，特别是其对细胞从玻璃表面脱离、解离成单细胞的活性以及对细胞增殖的抑制作用。使用L-929和CHO-K1株的成纤维细胞，以及HeLa-S3、JTC-16和RLC-10（2）株的上皮细胞。当将该蛋白酶加入到补充有胎牛血清的MEM中时，成纤维细胞被分离和解离。上皮细胞分离，但几乎没有解离。动力学数据获得有关的分散特性，这种细菌的中性蛋白酶连同几个方面的上皮细胞和成纤维细胞之间的差异，在细胞与细胞的性质，和细胞与基板的相互作用。
The effect of a bacterial neutral protease on cultured cells was examined with special reference to its activity on the detachment of cells from glass surface, dissociation into single cells and to the inhibitory effect on cell proliferation. Fibroblasts of strains L-929 and CHO-K1, and epithelial cells of strains HeLa-S3, JTC-16 and RLC-10(2) were employed. Fibroblasts were detached and dissociated when this protease was added to MEM supplemented with fetal bovine serum. The epithelial cells were detached but were scarcely dissociated. Kinetic data were obtained concerning the dispersing characteristics of this bacterial neutral protease together with a few aspects on the difference between epithelial cells and fibroblasts in the nature of cell to cell, and cell to substrate interactions.