A multimodal, multidimensional atlas of the C57BL/6J mouse brain
A multimodal, multidimensional atlas of the C57BL/6J mouse brain
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DOI：
10.1111/j.1469-7580.2004.00264.x
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发表时间：
2004-02-01
影响因子：
2.4
通讯作者：
Toga, AW
中科院分区：
文献类型：
--
作者：
MacKenzie-Graham, A;Lee, EF;Toga, AW
通过繁殖或破坏正常遗传途径，小鼠品系被广泛用于模拟人类疾病。图集通过提供比较标准，对于理解此类操作的影响提供了宝贵的帮助。我们开发了成年 C57BL/6J 小鼠大脑的数字图谱，作为存储和访问有关小鼠大脑的多种类型信息的综合框架。我们的实现是使用几种不同的成像技术构建的：磁共振显微镜、块面成像、经典组织学和免疫组织化学。除了原始图像和带注释的图像之外，它还包含数据库管理系统和一组用于比较来自不同技术的信息的工具。该框架通过建立小鼠大脑的规范表示并提供将独立数据插入与图谱相同空间的工具，可以轻松关联不同动物、研究人员或实验室的结果。该工具将有助于管理有关哺乳动物大脑的日益复杂和大量的信息。它提供了一个包含解剖成像背景下的遗传信息的框架，并为对基因型和表型之间的关系产生新的见解带来了巨大的希望。我们描述了一套工具，可以独立输入其他类型的数据、轻松检索信息和直接显示图像。因此，图谱成为管理小鼠大脑复杂遗传和表观遗传信息的框架。该图集和相关工具可通过 http://www.loni.ucla.edu/MAP 访问。
Strains of mice, through breeding or the disruption of normal genetic pathways, are widely used to model human diseases. Atlases are an invaluable aid in understanding the impact of such manipulations by providing a standard for comparison. We have developed a digital atlas of the adult C57BL/6J mouse brain as a comprehensive framework for storing and accessing the myriad types of information about the mouse brain. Our implementation was constructed using several different imaging techniques: magnetic resonance microscopy, blockface imaging, classical histology and immunohistochemistry. Along with raw and annotated images, it contains database management systems and a set of tools for comparing information from different techniques. The framework allows facile correlation of results from different animals, investigators or laboratories by establishing a canonical representation of the mouse brain and providing the tools for the insertion of independent data into the same space as the atlas. This tool will aid in managing the increasingly complex and voluminous amounts of information about the mammalian brain. It provides a framework that encompasses genetic information in the context of anatomical imaging and holds tremendous promise for producing new insights into the relationship between genotype and phenotype. We describe a suite of tools that enables the independent entry of other types of data, facile retrieval of information and straightforward display of images. Thus, the atlas becomes a framework for managing complex genetic and epigenetic information about the mouse brain. The atlas and associated tools may be accessed at http://www.loni.ucla.edu/MAP.