Serum amyloid A (SAA): influence on HDL-mediated cellular cholesterol efflux.
Serum amyloid A (SAA): influence on HDL-mediated cellular cholesterol efflux.
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发表时间：
1995-05
影响因子：
6.5
通讯作者：
C. Banka;T. Yuan;M. Beer;M. Kindy;L. Curtiss;F. D. Beer
中科院分区：
文献类型：
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作者：
C. Banka;T. Yuan;M. Beer;M. Kindy;L. Curtiss;F. D. Beer
正常高密度脂蛋白（N-HDL）在急性期（AP）反应期间通过血清淀粉样蛋白A（SAA）和载脂蛋白（apo）A-I的耗竭而重塑。为了确定这种重塑对HDL功能的影响，比较了N-HDL和AP-HDL与人单核细胞THP-1细胞相关并促进胆固醇流出的能力。与N-HDL相比，THP-1细胞优先结合AP-HDL。与THP-1细胞结合的AP-HDL颗粒的检查揭示了与起始材料的组成相比，apoSAA富集的、apoA-I耗尽的亚群的不成比例的关联。然而，N-HDL和AP-HDL促进胆固醇流出THP-1细胞同样有效，并以剂量依赖性的方式。当N-HDL与apoSAA进行实验性重塑，以实现类似于优先结合颗粒的载脂蛋白组合物时，细胞胆固醇流出减少了30%。只有当apoSAA占HDL蛋白的50%以上时，急性期反应中apoSAA对HDL的重塑才能改变胆固醇流出。
Normal high density lipoprotein (N-HDL) is remodeled during acute phase (AP) reactions by the association of serum amyloid A (SAA) and the depletion of apolipoprotein (apo) A-I. To determine the impact of this remodeling on HDL function, the capacities of N-HDL and AP-HDL to associate with and promote cholesterol efflux from human monocytic THP-1 cells were compared. THP-1 cells preferentially bound AP-HDL compared with N-HDL. Examination of the AP-HDL particles bound to THP-1 cells revealed a disproportionate association of an apoSAA-enriched, apoA-I-depleted subpopulation compared with the composition of the starting material. However, N-HDL and AP-HDL promoted cholesterol efflux from THP-1 cells equally efficiently and in a dose-dependent manner. When N-HDL was experimentally remodeled with apoSAA to achieve an apoprotein composition similar to that of the preferentially bound particles, cellular cholesterol efflux was reduced by 30%. The remodelling of HDL with apoSAA during the acute phase reaction alters cholesterol efflux only when apoSAA constitutes more than 50% of the HDL protein.