COCLUSTERING OF CD4 (L3T4) MOLECULE WITH THE T-CELL RECEPTOR IS INDUCED BY SPECIFIC DIRECT INTERACTION OF HELPER T-CELLS AND ANTIGEN-PRESENTING CELLS
COCLUSTERING OF CD4 (L3T4) MOLECULE WITH THE T-CELL RECEPTOR IS INDUCED BY SPECIFIC DIRECT INTERACTION OF HELPER T-CELLS AND ANTIGEN-PRESENTING CELLS
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DOI：
10.1073/pnas.84.16.5888
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发表时间：
1987-08-01
影响因子：
11.1
通讯作者：
SWAIN, SL
中科院分区：
文献类型：
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作者：
KUPFER, A;SINGER, SJ;SWAIN, SL
用单抗阻断的研究表明，辅助T细胞的识别和触发涉及到CD4(L3T4)辅助分子以及与T3复合体相连的T细胞受体(TCR)。我们研究了克隆的小鼠辅助性T细胞系与抗原呈递的B细胞系直接相互作用过程中L3T4和TCR的表面分布。利用免疫荧光显微镜，我们发现在两个细胞之间形成的1：1细胞对中，L3T4和TCR重新分布在T细胞表面，从而集中在细胞-细胞接触区。只有当相关的抗原和适当的主要组织相容性II类分子被提呈给T细胞时，L3T4和TCR才会发生这种共同聚集，因此它需要TCR与其在抗原提呈细胞上的复杂配体的特异性相互作用。
Blocking studies with monoclonal antibodies have suggested that helper T cell recognition and triggering involve the CD4 (L3T4) accessory molecule as well as the T-cell receptor (TCR) that is linked to the T3 complex. We have investigated the surface distribution of L3T4 and TCR during the direct interaction of a cloned murine helper T-cell line with an antigen-presenting B-cell line. Using immunofluorescence microscopy, we show that in 1:1 cell couples formed between the two cells, in which a specific interaction can be demonstrated, the L3T4 and the TCR become redistributed on the T-cell surface so that they are concentrated in the cell-cell contact region. This coclustering of L3T4 with TCR occurs only when the relevant antigen and appropriate major histocompatibility class II molecules are presented to the T cell, and it therefore requires the specific interaction of the TCR with its complex ligand on the antigen-presenting cell.