A mitochondria-targeted fluorescent probe for fast detecting hypochlorite in living cells
A mitochondria-targeted fluorescent probe for fast detecting hypochlorite in living cells
复制标题
一种用于快速检测活细胞中次氯酸盐的线粒体靶向荧光探针
DOI：
10.1016/j.dyepig.2020.108192
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发表时间：
2020-05-01
影响因子：
4.5
通讯作者：
Zhou, Xin
中科院分区：
文献类型：
--
作者：
Li, Hongfang;Miao, Yu;Zhou, Xin
设计并合成了一种快速响应的荧光探针PI-Py，用于检测次氯酸根（ClO-）。由于吩噻嗪部分的PET效应，探针PI-Py本质上是非荧光的。在与ClO-反应后，PET效应被阻断，并且BODIPY单元的强绿色发射被恢复。与其它活性氧相比，探针PI-Py对ClO-具有很好的选择性。计算出ClO-的检测限为1.7 nM，证明该探针具有高灵敏度。此外，PI-Py探针已被证明是有效的可视化外源ClO-在活的HeLa细胞中的变化。采用Mito Tracker red的共定位研究揭示了PI-Py特异性地位于线粒体中。
A fast-responsive fluorescent probe PI-Py was designed and synthesized for sensitive and selective detecting hypochlorite (ClO-). The probe PI-Py was intrinsically non-fluorescent due to the PET effect of the phenothiazine moiety. After reacting with ClO-, the PET effect was blocked, and a strong green emission of the BODIPY unit was recovered. The probe PI-Py showed an excellent selectivity towards ClO- in comparison of other ROS. The limit of detection for ClO- was calculated to be 1.7 nM, proving the probe's high sensitivity. Moreover, the probe PI-Py has been proved to be effective in visualizing the variations of exogenous ClO- in living HeLa cells. A colocalization study employing Mito Tracker red reveals that the PI-Py is specifically located in the mitochondria.