MONOCLONAL-ANTIBODIES SPECIFIC FOR SICKLE-CELL HEMOGLOBIN
MONOCLONAL-ANTIBODIES SPECIFIC FOR SICKLE-CELL HEMOGLOBIN
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DOI：
10.3109/03630268508997010
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发表时间：
1985-01-01
期刊：
影响因子：
1
通讯作者：
STANKER, LH
中科院分区：
文献类型：
--
作者：
JENSEN, RH;VANDERLAAN, M;STANKER, LH
Two mouse hybridoma cell lines were isolated which produce monoclonal antibodies that bind hemoglobin S. The mice were immunized with peptide-protein conjugates to stimulate a response to the amino terminal peptide of the beta chain of hemoglobin S, where the single amino acid difference between A and S occurs. Immunocharacterization of the antibodies shows that they bind specifically to the immunogen peptide and to hemoglobin S. The specificity for S is high enough that one AS cell in a mixture with a million AA cells is labeled by antibody, and such cells can be analyzed by flow cytometry. Immunoblotting of electrophoretic gels allows definitive identification of hemoglobin S as compared with other hemoglobins with similar electrophoretic mobility.
