Electroacupuncture downregulates TLR2/4 and pro-inflammatory cytokine expression after surgical trauma stress without adrenal glands involvement
Electroacupuncture downregulates TLR2/4 and pro-inflammatory cytokine expression after surgical trauma stress without adrenal glands involvement
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DOI：
10.1016/j.brainresbull.2009.04.020
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发表时间：
2009-08-28
影响因子：
3.8
通讯作者：
Wu, Gen-Cheng
中科院分区：
文献类型：
--
作者：
Wang, Jun;Zhao, Hui;Wu, Gen-Cheng
越来越多的证据表明，电针具有调节免疫功能的作用，但其机制尚需进一步研究。本研究旨在探讨电针对创伤应激后Toll样受体2和4(TLR2/TLR4)及促炎细胞因子表达的影响。用实时定量聚合酶链式反应检测TLR2/4和促炎细胞因子的mRNA水平。用ELISA法检测TLR2/TLR4蛋白表达，用Western印迹法检测促炎症细胞因子的产生。结果表明，手术创伤应激使小鼠脾组织TLR2 mRNA和TLR2/4蛋白表达增加，促炎症细胞因子(如IL-1β)mRNA和蛋白表达增强。这些影响可能会因肾上腺切除术(ADX)而恶化。电针足三里穴可显著抑制手术创伤小鼠脾组织TLR2 mRNA和TLR2/4蛋白的表达以及脾和血浆中促炎症细胞因子的表达。但阿昔洛韦不能阻断电针的作用。这些结果表明，手术创伤应激启动了先天免疫系统，促进了TLR2的表达和促炎细胞因子的产生。在外科创伤应激模型中，电针抑制TLR2/4和促炎细胞因子产生抗炎作用，而不涉及肾上腺。(C)2009年，爱思唯尔公司出版。
Cumulative evidences suggest that electroacupuncture (EA) can modulate immune function, but the mechanism needs further study. In the present study, the contribution of EA on toll-like receptors 2 and 4 (TLR2/TLR4) and pro-inflammatory cytokine expression after surgical trauma stress were investigated. The mRNA level of both TLR2/4 and pro-inflammatory cytokine was measured by quantitative real-time PCR. ELISA and Western blot assay were chosen for TLR2/TLR4 protein expression and pro-inflammatory cytokine production, respectively. The results showed that surgical trauma stress increased TLR2 mRNA and TLR2/4 proteins in the spleen and augmented pro-inflammatory cytokines (e.g. IL-1 beta) mRNA and protein expression in the spleen and plasma. These effects could be deteriorated by adrenalectomy (ADX). EA at "Zusanli" acupoint significantly inhibited surgical trauma-induced TLR2 mRNA and TLR2/4 protein expression in spleen and pro-inflammatory cytokine expression in the spleen and plasma. ADX, however, could not block the effect of EA. These results suggested that surgical trauma stress primes the innate immune system for enhanced TLR2 expression and pro-inflammatory cytokine production. EA inhibits TLR2/4 and pro-inflammatory cytokines to produce an anti-inflammatory effect in a surgical trauma stress model, without adrenal gland involvement. (C) 2009 Published by Elsevier Inc.