EXPRESSION PATTERNS OF THE HOMEO BOX-CONTAINING GENES EN-1 AND EN-2 AND THE PROTO-ONCOGENE INT-1 DIVERGE DURING MOUSE DEVELOPMENT
EXPRESSION PATTERNS OF THE HOMEO BOX-CONTAINING GENES EN-1 AND EN-2 AND THE PROTO-ONCOGENE INT-1 DIVERGE DURING MOUSE DEVELOPMENT
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DOI：
10.1101/gad.2.12b.1736
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发表时间：
1988-12-01
影响因子：
10.5
通讯作者：
JOYNER, AL
中科院分区：
文献类型：
--
作者：
DAVIS, CA;JOYNER, AL
我们已经比较了小鼠基因En-1，En-2，和int-1在发展过程中的原位杂交的表达。所有这三个基因的表达，首先检测到在8.0天的重叠带的前神经褶皱。到12.0天，表达模式出现分歧。En-1和En-2在中枢神经系统（CNS）中脑/后脑交界处的类似细胞环中表达。En-1也表达从头在两个横向条纹延伸长度的后脑和脊髓，在发展中的脊柱，在两个横向条纹的皮节衍生的细胞，并在尾巴和肢芽。到12.0天，int-1表达与En基因几乎没有重叠，并且在后期不能检测到。在15.5天，En基因表达主要限于中脑/后脑中重叠但不相同的分化细胞。在成人中，En-1和En-2在脑桥中标记相同的细胞组，但En-2单独在小脑的颗粒层中检测到。这些结果与int-1和En基因在发育早期在CNS中定义空间结构域中发挥作用一致。在发育后期，En基因可能在神经发生中具有额外的功能。En-1在发育中的脊索周围管中的表达表明它也可能参与椎骨组装。
We have compared the expression of the murine genes En-1,En-2, and int-1 during development by in situ hybridization. Expression of all three genes was first detected at 8.0 days in overlapping bands of the anterior neural folds. By 12.0 days the expression patterns diverged. En-1 and En-2 were expressed in a similar ring of cells in the central nervous system (CNS) at the midbrain/hindbrain junction. En-1 was also expressed de novo in two lateral stripes extending the length of the hindbrain and spinal cord, in the developing vertebral column, in two lateral stripes of dermatome-derived cells, and in the tail and limb buds. By 12.0 days int-1 expression showed little overlap with the En genes and could not be detected at later stages. At 15.5 days En gene expression was primarily limited to the midbrain/hindbrain in overlapping but nonidentical sets of differentiated cells. In the adult, En-1 and En-2 marked the same sets of cells in the pons, but En-2 alone was detected in the granular layer of the cerebellum. The results are consistent with int-1 and the En genes playing a role early in development in defining spatial domains in the CNS. Later in development the En genes may have an additional function in neurogenesis. En-1 expression in the developing pericordal tube suggests that it may also be involved in vertebral assembly.