Differential expression of connexins in trigeminal ganglion neurons and satellite glial cells in response to chronic or acute joint inflammation.
Differential expression of connexins in trigeminal ganglion neurons and satellite glial cells in response to chronic or acute joint inflammation.
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DOI：
10.1017/s1740925x09990093
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发表时间：
2008-11
影响因子：
--
通讯作者：
Durham PL
中科院分区：
文献类型：
--
作者：
Garrett FG;Durham PL
已显示响应于炎症刺激的三叉神经激活通过三叉神经节中的间隙连接增加神经元-胶质细胞通信。本研究的目的是确定急性或慢性关节炎症反应中三叉神经节间隙连接蛋白（Cxs）表达的变化。虽然在基础条件下检测到Cxs 26，36，40和43的mRNA，但在辣椒素或完全弗氏佐剂（CFA）注射到颞下颌关节（TMJ）后，仅Cxs 26，36和40的蛋白表达增加。虽然神经元和卫星胶质细胞之间的Cx 26斑块形成短暂增加辣椒素注射后，神经元和卫星胶质细胞之间的Cx 26斑块形成持续响应CFA。有趣的是，Cx 36和Cx40的水平仅在辣椒素或CFA注射后的神经元中升高，但时间响应与观察到的Cx 26相似。与此相反，Cx43的表达没有增加，在神经元或卫星神经胶质细胞在CFA或辣椒素。因此，三叉神经节神经元和卫星神经胶质细胞可以差异调节Cx表达的类型和持续时间的炎症刺激，这可能有助于增加神经元-神经胶质细胞的通信在急性和慢性炎症和疼痛的颞下颌关节。
Trigeminal nerve activation in response to inflammatory stimuli has been shown to increase neuron–glia communication via gap junctions in trigeminal ganglion. The goal of this study was to identify changes in the expression of gap junction proteins, connexins (Cxs), in trigeminal ganglia in response to acute or chronic joint inflammation. Although mRNA for Cxs 26, 36, 40 and 43 was detected under basal conditions, protein expression of only Cxs 26, 36 and 40 increased following capsaicin or complete Freund’s adjuvant (CFA) injection into the temporomandibular joint (TMJ). While Cx26 plaque formation between neurons and satellite glia was transiently increased following capsaicin injections, Cx26 plaque formation between neurons and satellite glia was sustained in response to CFA. Interestingly, levels of Cx36 and Cx40 were only elevated in neurons following capsaicin or CFA injections, but the temporal response was similar to that observed for Cx26. In contrast, Cx43 expression was not increased in neurons or satellite glial cells in response to CFA or capsaicin. Thus, trigeminal ganglion neurons and satellite glia can differentially regulate Cx expression in response to the type and duration of inflammatory stimuli, which likely facilitates increased neuron–glia communication during acute and chronic inflammation and pain in the TMJ.