MYXOVIRUS REPLICATION IN CHICKEN EMBRYOS CAN BE SUPPRESSED BY APROTININ DUE TO THE BLOCKAGE OF VIRAL GLYCOPROTEIN CLEAVAGE
MYXOVIRUS REPLICATION IN CHICKEN EMBRYOS CAN BE SUPPRESSED BY APROTININ DUE TO THE BLOCKAGE OF VIRAL GLYCOPROTEIN CLEAVAGE
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DOI：
10.1099/0022-1317-66-7-1633
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发表时间：
1985-01-01
影响因子：
3.8
通讯作者：
BUKRINSKAYA, AG
中科院分区：
文献类型：
--
作者：
ZHIRNOV, OP;OVCHARENKO, AV;BUKRINSKAYA, AG
将蛋白酶抑制剂抑肽酶注射到低感染复数感染的含胚卵的尿囊腔中。与不同的流感病毒和副粘病毒一起使用可显着减少至少 100 倍的增殖。在这些条件下，产生的大多数病毒颗粒是非感染性的，并且含有未切割的糖蛋白，这可能是由于抑肽酶抑制蛋白酶活性所致。
Injection of the protease inhibitor, aprotinin, into the allantoic cavity of embryonated eggs infected at low m.o.i. with different influenza viruses and paramyxoviruses markedly reduced multiplication by at least 100-fold. Under these conditions, most viral particles produced were non-infectious and contained uncleaved glycoproteins, presumably resulting from aprotinin suppression of protease activity.