Sulphate reduction by aggregates of sulphate-reducing bacteria and homo-acetogenic bacteria in a lab-scale gas-lift reactor
Sulphate reduction by aggregates of sulphate-reducing bacteria and homo-acetogenic bacteria in a lab-scale gas-lift reactor
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DOI：
10.1016/0960-8524(95)00117-4
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发表时间：
1995
影响因子：
11.4
通讯作者：
Renze T van Houten;S. O. Elferink;Sandra E. van Hamel;L. Pol;G. Lettinga
中科院分区：
文献类型：
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作者：
Renze T van Houten;S. O. Elferink;Sandra E. van Hamel;L. Pol;G. Lettinga
研究了气举反应器中硫酸盐生物还原的微生物学特性。氢和二氧化碳被用作能源和碳源。通过浮石颗粒的聚集和自然固定化获得生物质保留。H2/ co2的生物硫酸盐还原在pH为5.5 ~ 8.0的范围内适用，在pH为7.5附近时效果最佳。pH值影响聚集体的形态和直径。pH为7.0时，团聚体的索氏平均直径为1.5 mm。此外，相衬显微镜和扫描电镜显示高度分枝的聚集体表面。pH值增加导致表面不规则性增加，但不影响颗粒直径。pH降低导致表面不规则性降低，骨料平均直径由pH 7.0时的1.50 mm变为pH 5.5时的2.26 mm。pH值对生物量组成的影响不显著。通过相衬显微镜、扫描电镜和富集检查，发现在所有pH值下，Desulfovibrio和Acetobacterium是最丰富的微生物。
Microbiological aspects of biological sulphate reduction in gas-lift reactors were studied. Hydrogen and carbon dioxide were used as energy and carbon sources. Biomass retention was obtained by aggregation and natural immobilization on pumice particles. Biological sulphate reduction on H2/CO2appeared to be applicable within a pH range of 5·5–8·0 with an optimum near pH 7·5. The pH affected aggregate configuration and diameter. At pH 7·0, the average Sauter mean diameter of the aggregates was 1·5 mm. Moreover, phase-contrast and SEM microscopy showed highly branched aggregate surfaces. A pH increase led to increased surface irregularity without affecting the particle diameter. A pH decrease caused a decreased surface irregularity and changed the aggregate Sauter mean diameter from 1·50 mm at pH 7·0 to 2·26 at pH 5·5. However, the pH did not have a significant effect on the biomass composition. Examination of the bacterial composition of the aggregates by phasecontrast microscopy, SEM microscopy, as well as enrichments, showed that at all pH values Desulfovibrio species and Acetobacterium species were the most abundant micro-organisms.