Genome-wide identification, phylogeny and expression analysis of the lipoxygenase gene family in cucumber
Genome-wide identification, phylogeny and expression analysis of the lipoxygenase gene family in cucumber
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黄瓜脂氧合酶基因家族的全基因组鉴定、系统发育和表达分析
DOI：
10.4238/2011.october.25.9
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发表时间：
2011-01-01
影响因子：
0.4
通讯作者：
Jiang, L. W.
中科院分区：
文献类型：
--
作者：
Liu, S. Q.;Liu, X. H.;Jiang, L. W.
植物脂氧合酶（LOX）通过生物合成调节分子和对病原体、伤害和胁迫的防御反应，参与植物生长发育的控制过程。迄今为止，关于黄瓜（Cucumis sativus L.） LOX蛋白和组织表达谱的详细报道很少。最近完成的黄瓜基因组序列现在允许对黄瓜的LOX基因家族进行全基因组分析，并与拟南芥和水稻的LOX进行比较。我们在黄瓜基因组中鉴定出23个候选LOX基因；系统发育分析表明，这些LOX成员聚为两组，称为1型和2型，这与先前的研究结果一致。序列分析表明，在黄瓜LOX蛋白中，铁的5个结合位点高度保守，其中包括LOX结构域的2个共识组氨酸。染色体定位和基因组分布分析表明，串联重复和/或多倍体重复促进了黄瓜LOX基因家族的扩增。内含子/外显子结构分析表明，单子代和双子代的祖先中只存在少数现存的内含子模式。表达数据显示，黄瓜LOX基因家族在植物组织中分布广泛，表明其在不同组织中发挥着不同的功能。
Plant lipoxygenase (LOX) is involved in growth and developmental control processes, through the biosynthesis of regulatory molecules and defense responses to pathogens, wounding and stress. To date, few LOX proteins and little tissue expression profiling have been reported in detail for cucumber (Cucumis sativus L.). Recent completion of the cucumber genome sequence now permits genome-wide analysis of the LOX gene family in cucumber as well as comparison with LOX in Arabidopsis and rice. We identified 23 candidate LOX genes in the cucumber genome; phylogenetic analysis indicated that these LOX members cluster into two groups, designated types 1 and 2, as expected from previous studies. Sequence analysis showed that five binding sites of iron, including two consensus histidines in the LOX domain, are highly conserved in the cucumber LOX proteins. Analysis of chromosomal localization and genome distribution suggested that tandem duplication and/or polyploidal duplication contributed to the expansion of the cucumber LOX gene family. Based on intron/exon structure analysis, only a few of the extant intron patterns existed in the ancestor of monocots and eudicots. Expression data showed widespread distribution of the cucumber LOX gene family within plant tissues, suggesting that they perform different functions in different tissues.