Efficacy of a doxycycline treatment regimen initiated during three different phases of experimental ehrlichiosis.
Efficacy of a doxycycline treatment regimen initiated during three different phases of experimental ehrlichiosis.
复制标题
在实验性埃利希体病的三个不同阶段开始的强力霉素治疗方案的疗效。
DOI：
10.1128/aac.01622-09
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发表时间：
2010
影响因子：
4.9
通讯作者：
Stich,RogerW
中科院分区：
文献类型：
--
作者：
McClure,JenniferC;Crothers,MichelleL;Schaefer,JohnJ;Stanley,PatrickD;Needham,GlenR;Ewing,SA;Stich,RogerW
多西环素是治疗犬单核细胞埃里希体病（CME）的首选药物，CME是一种特征明确的疾病，也是蜱传人畜共患病的有价值模型。多西环素治疗后清除犬埃里希体的报告表明，开始治疗的疾病阶段影响这些治疗的结果。本研究的目的是评价28天强力霉素方案清除实验性E的疗效。在疾病的三个阶段接受治疗的狗的感染。10只家犬弗罗姆.在CME的急性、亚临床或慢性阶段，犬携带者和用强力霉素治疗。每天监测每只犬的直肠温度，每半周采集一次血样，并采集每只犬的血红扇头蜱进行异种诊断。从急性或亚临床CME期间接受治疗的犬中采集的血液对E呈PCR阴性。犬的临床参数得到改善，但从慢性CME期间治疗的犬采集的血样仍间歇性PCR阳性。用强力霉素处理后的狗喂养的血蜱对E.犬，无论何时开始治疗。然而，较少的蜱成为PCR阳性后，喂养两个持续感染的狗治疗多西环素，然后利福平，这表明抗生素治疗可以减少蜱收购E。犬。
Doxycycline is the treatment of choice for canine monocytic ehrlichiosis (CME), a well-characterized disease and valuable model for tick-borne zoonoses. Conflicting reports of clearance ofEhrlichia canisafter treatment with doxycycline suggested that the disease phase during which treatment is initiated influences outcomes of these treatments. The purpose of this study was to evaluate the efficacy of a 28-day doxycycline regimen for clearance of experimentalE. canisinfections from dogs treated during three phases of the disease. Ten dogs were inoculated with blood fromE. caniscarriers and treated with doxycycline during acute, subclinical, or chronic phases of CME. Daily rectal temperatures and semiweekly blood samples were monitored from each dog, andRhipicephalus sanguineusticks were acquisition fed on each dog for xenodiagnosis. Blood collected from dogs treated during acute or subclinical CME became PCR negative forE. canisas clinical parameters improved, but blood samples collected from dogs treated during chronic CME remained intermittently PCR positive.R. sanguineusticks fed on dogs after doxycycline treatments became PCR positive forE. canis, regardless of when treatment was initiated. However, fewer ticks became PCR positive after feeding on two persistently infected dogs treated with doxycycline followed by rifampin, suggesting that antibiotic therapy can reduce tick acquisition ofE. canis.