Molecular compatibility and renal graft survival--the HLA DRB1 genotyping.
Molecular compatibility and renal graft survival--the HLA DRB1 genotyping.
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分子相容性和肾移植物存活——HLA DRB1 基因分型。
DOI：
10.1097/00007890-199302000-00030
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发表时间：
1993
期刊：
影响因子：
6.2
通讯作者：
M. Trucco
中科院分区：
文献类型：
--
作者：
S. Hsia;J. Y. Tong;G. Parris;D. Nghiem;E. Cottington;W. Rudert;M. Trucco
HLA-DRB 1等位基因类型的确定，通过使用序列特异性寡核苷酸分析91个肾供受者对，随访30个月。采用Kaplan-Meier法评估匹配组和不匹配组的移植物存活率（GS）。通过等位基因类型匹配和配对的错配来测量相容性程度。此外，将HLA DR基因型分为5组，即DR 1、DR 2、DR 4、也具有DRw 52等位基因的组以及DR 7、DR 9和DR 10的组。一组中的所有类型都被认为是匹配的。还包括同一研究组的血清型进行比较。观察到上级GS与相容的DRB 1等位基因类型、血清型和分组的DRB 1类型的关联。HLA DRB 1基因型以组配型分析的GS率显著高于以型配型分析的GS率（P = 0.03）（P > 0.34）。由于可以基于核苷酸序列变异分配许多等位基因类型，因此需要大量的供体-受体对来获得统计学上显著的结果。
HLA DRB1 allelic types were determined by using sequence-specific oligonucleotides for the analysis of 91 renal donor-recipient pairs that were followed for 30 months. The Kaplan-Meier method was used to evaluate graft survivals (GS) of the matched and mismatched groups. The degree of compatibility was measured by allelic type matches and mismatches of the pairs. Furthermore, HLA DR genotypes were categorized into five groups, namely DR1, DR2, DR4, the group that also has the DRw52 allele, and the group of DR7, DR9, and DR10. All types within a group were considered a match. Serotypes of the same study group were also included for comparison. Associations of superior GS with compatible DRB1 allelic types, serotypes, and grouped DRB1 types were observed. A significantly higher GS rate was found in donor-recipient pairs when HLA DRB1 types were analyzed by group mismatching (P = 0.03) rather than type mismatching (P > 0.34). Because of the numerous allelic types that can be assigned on the basis of nucleotide sequence variations, a larger number of donor-recipient pairs is required to derive statistically significant results.