The sequence of an atriopeptigen: a precursor of the bioactive atrial peptides.
The sequence of an atriopeptigen: a precursor of the bioactive atrial peptides.
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心房肽原的序列：生物活性心房肽的前体。
DOI：
10.1016/0006-291x(84)90749-6
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发表时间：
1984
影响因子：
3.1
通讯作者：
P. Needleman
中科院分区：
文献类型：
--
作者：
D. Geller;M. Currie;N. Siegel;K. Fok;S. Adams;P. Needleman
从大鼠心房提取物中提取高分子量组分（心房肽原-APG），经等电聚焦和反相高效液相色谱分离，得到纯的APG。纯化溴化氰裂解物的粗高分子量部分导致分离出单一的生物活性溴化氰裂解肽。这些肽的序列分析与最近报道的中等分子量心房肽的序列分析相结合（Thibault等，（1984）FEBS Letters 167，352-356，和Kangwa等，生物化学、生物物理Res. Commun 119，933-940）提供了111个残基APG的完整一级结构。
The high molecular weight fraction (atriopeptigen-APG) obtained by gel filtration chromatography of rat atrial extracts was fractionated by isoelectric focusing and reverse phase HPLC to obtain a pure APG. Purification of cyanogen bromide digests of the crude high molecular weight fraction resulted in the isolation of a single biologically active cyanogen bromide cleavage peptide. Sequence analyses of these peptides coupled with recent reports of sequence analyses of intermediate molecular weight atrial peptides (Thibault, et al. (1984) FEBS Letters 167, 352–356, and Kangwa, et al., Biochem. Biophys. Res. Commun 119, 933–940) provide the complete primary structure of an 111 residue APG.