Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element
Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element
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在粟酒裂殖酵母中复制质粒：通过新的遗传元件改善对称分离
DOI：
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发表时间：
1986
影响因子：
5.3
通讯作者：
J. Kohli
中科院分区：
文献类型：
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作者：
W. Heyer;M. Sipiczki;J. Kohli
我们在裂糖酵母（Schizosaccharomyces pombe）中鉴定了一些广泛使用的酵母-大肠杆菌穿梭载体。2微米载体pDB248和YEp13在S. pombe中表现出高转化频率、中等有丝分裂和低减数分裂稳定性以及低拷贝数，类似于它们在酿酒酵母[cir0]菌株中的行为。酿酒葡萄球菌整合载体pLEU2和pURA3以非常低的频率转化S. pombe，但令人惊讶的是，以非整合的方式。相反，它们是自主复制的，并且它们显示出非常高的拷贝数（每个含有质粒的细胞多达150个拷贝）。这可能反映了S. pombe质粒DNA复制缺乏序列特异性。研究了pombe S. pombe ars载体pFL20及其衍生的一系列质粒，分析了该质粒异常高的稳定性。通过对转化的pombe细胞进行系谱分析，测定了质粒的分裂稳定性和分裂情况。在有丝分裂和减数分裂过程中，通过改善质粒分配，鉴定出一段pombe S. DNA片段（stb）来稳定pFL20。
We characterized a number of widely used yeast-Escherichia coli shuttle vectors in the fission yeast Schizosaccharomyces pombe. The 2 micron vectors pDB248 and YEp13 showed high frequency of transformation, intermediate mitotic and low meiotic stability, and a low copy number in S. pombe, analogous to their behavior in [cir0] strains of Saccharomyces cerevisiae. The S. cerevisiae integration vectors pLEU2 and pURA3 transformed S. pombe at very low frequencies but, surprisingly, in a nonintegrative fashion. Instead, they replicated autonomously, and they showed very high copy numbers (up to 150 copies per plasmid-containing cell). This could reflect a lack of sequence specificity for replication of plasmid DNA in S. pombe. pFL20, an S. pombe ars vector, and a series of plasmids derived from it were studied to analyze the unusually high stability of this plasmid. Mitotic stability and partitioning of the plasmids was measured by pedigree analysis of transformed S. pombe cells. An S. pombe DNA fragment (stb) was identified that stabilizes pFL20 by improvement of plasmid partitioning in mitosis and meiosis.
酵母中不稳定质粒的复制和分离。
影响因子：
2.6
作者：
Zakian,VA;Kupfer,DM
通讯作者：
Kupfer,DM
裂殖酵母裂殖酵母乙醇脱氢酶基因的一级结构。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Russell,PR;Hall,BD
通讯作者：
Hall,BD