Protein oxidation and aggregation in UVA-irradiated Escherichia coli cells as signs of accelerated cellular senescence
Protein oxidation and aggregation in UVA-irradiated Escherichia coli cells as signs of accelerated cellular senescence
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DOI：
10.1111/j.1462-2920.2010.02268.x
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发表时间：
2010-11-01
影响因子：
5.1
通讯作者：
Egli, Thomas
中科院分区：
文献类型：
--
作者：
Bosshard, Franziska;Riedel, Kathrin;Egli, Thomas
P>太阳能消毒（SODIS）是一种简单的饮用水处理方法，可以改善其他方法无法实现的微生物水质。它利用太阳辐射对病原微生物和病毒的有害作用。多项流行病学研究已记录了对健康的积极影响。然而，这种简单治疗过程中损害细胞的分子机制尚未完全了解。在这里，我们表明蛋白质损伤在阳光失活过程中至关重要。已通过羰基化蛋白质的免疫印迹方法和基于半定量蛋白质组学的聚集测定法评估了 UVA 照射的大肠杆菌细胞中的蛋白质损伤。细胞内多种结构蛋白和酶蛋白受到羰基化和聚集的影响。 UVA 照射的目标是转录和翻译装置、运输系统、氨基酸合成和降解、呼吸、ATP 合成、糖酵解、TCA 循环、伴侣功能和过氧化氢酶等重要细胞功能。 SODIS 引起的蛋白质损伤模式与活性氧应激引起的模式非常相似。因此，阳光可能会加速细胞衰老并导致 UVA 照射的细胞失活并最终死亡。
P>Solar disinfection (SODIS) is a simple drinking water treatment method that improves microbiological water quality where other means are unavailable. It makes use of the deleterious effect of solar irradiation on pathogenic microbes and viruses. A positive impact on health has been documented in several epidemiological studies. However, the molecular mechanisms damaging cells during this simple treatment are not yet fully understood. Here we show that protein damage is crucial in the process of inactivation by sunlight. Protein damages in UVA-irradiated Escherichia coli cells have been evaluated by an immunoblot method for carbonylated proteins and an aggregation assay based on semi-quantitative proteomics. A wide spectrum of structural and enzymatic proteins within the cell is affected by carbonylation and aggregation. Vital cellular functions like the transcription and translation apparatus, transport systems, amino acid synthesis and degradation, respiration, ATP synthesis, glycolysis, the TCA cycle, chaperone functions and catalase are targeted by UVA irradiation. The protein damage pattern caused by SODIS strongly resembles the pattern caused by reactive oxygen stress. Hence, sunlight probably accelerates cellular senescence and leads to the inactivation and finally death of UVA-irradiated cells.