Molecular epidemiology of rotavirus diarrhea among children and adults in Nepal: Detection of G12 strains with P[6] or P[8] and a G11P[25] strain
Molecular epidemiology of rotavirus diarrhea among children and adults in Nepal: Detection of G12 strains with P[6] or P[8] and a G11P[25] strain
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DOI：
10.1128/jcm.01089-06
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发表时间：
2006-10-01
影响因子：
9.4
通讯作者：
Nakagomi, Osamu
中科院分区：
文献类型：
--
作者：
Uchida, Ryuichi;Pandey, Basu Dev;Nakagomi, Osamu
在尼泊尔轮状病毒疫苗的预期中，进行了这项研究，以确定轮状病毒的G和P血清型和电泳型的分布，以检查是否有任何新出现的血清型或不寻常的菌株在尼泊尔儿童和成人中传播。在1，315例腹泻粪便标本中，通过酶联免疫吸附试验在666例5岁以下患者中的116例（17%）、260例5至14岁患者中的18例（7%）和358例15岁及以上患者中的19例（5%）中检测到轮状病毒。大约75%的轮状病毒腹泻发生在5岁以下的儿童中。在三个年龄组中发现的轮状病毒中，约70%属于血清型GIP[8]。有趣的是，有29（20%）G12轮状病毒携带P[8]或P[6]，1（0.7%）G11轮状病毒携带不寻常的P[25]基因型。RNA聚丙烯酰胺凝胶电泳可区分出19个菌株（电泳型），其中有3个共显性菌株带有GIP[8]和长RNA带型。在G12轮状病毒中可区分出5种电泳型，它们都具有长RNA模式。事实上，20%的轮状病毒是携带P[8]或P[6]的G12毒株，并且具有多种电泳型，这表明G12毒株不是更罕见的毒株，但它们对当前和未来的疫苗构成了新的挑战。电泳分型所确定的多个毒株的存在表明尼泊尔轮状病毒基因库的丰富性，不寻常的毒株可能会继续出现。
In anticipation of a rotavirus vaccine in Nepal, this study was undertaken to determine the distribution of the G and P serotypes and electropherotypes of rotaviruses in order to examine if there is any emerging serotype or unusual strain circulating in children and adults in Nepal. Of 1,315 diarrheal stool specimens, rotavirus was detected by an enzyme-linked immunosorbent assay in 116 (17%) of 666 patients less than 5 years of age, in 18 (7%) of 260 patients 5 to 14 years of age, and in 19 (5%) of 358 patients 15 years of age and older. Approximately 75% of rotavirus diarrhea occurred in children less than 5 years of age. Approximately 70% of rotaviruses found in each of the three age groups belonged to serotype GIP[8]. Interestingly, there were 29 (20%) G12 rotaviruses carrying either P[8] or P[6] and one (0.7%) G11 rotavirus carrying an unusual P[25] genotype. RNA polyacrylamide gel electrophoresis discriminated 19 strains (electropherotypes), among which there were three codominant strains carrying GIP[8] and long RNA patterns. Five electropherotypes were discriminated among G12 rotaviruses, all of which had long RNA patterns. The fact that 20% of rotaviruses were G12 strains carrying either P[8] or P[6] and had multiple electropherotypes suggest that G12 strains are not more rare strains but that they pose an emerging challenge to current and future vaccines. The presence of multiple strains as defined by electropherotypes suggests the richness of the rotavirus gene pool in Nepal, where unusual strains may continue to emerge.