Phototaxis and photophobic responses in Stentor coeruleus. Action spectrum and role of Ca2+ fluxes.
Phototaxis and photophobic responses in Stentor coeruleus. Action spectrum and role of Ca2+ fluxes.
复制标题
Stentor coeruleus 的趋光性和畏光反应。
DOI：
10.1016/0304-4165(84)90274-5
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Häder,M
中科院分区：
文献类型：
--
作者：
Kim,IH;Prusti,RK;Song,PS;Häder,DP;Häder,M
本文研究了纤毛肌蓝斑的负趋光性取向、递增畏光反应和光诱导动作电位。基于注入率-响应曲线的分辨作用光谱与作为光感受器的stentorin是一致的。钙通量阻滞剂延长纤毛停止和逆转的反应时间，并抑制增强的畏光反应。据信影响膜结合钙泵的药物同样会抑制恐惧症反应。另一方面，α-磷脂酸促进Ca~(2+)内流并增强生物体对光的敏感性，从而为Ca~(2+)内流的作用提供明确的证据。响应时间的变化降低了趋光性取向的程度，表明这种生物体中的负趋光性是由个别纤毛随后的恐惧反应引起的，因为当有机体在侧光中向前移动时，相关的光感受器颗粒经历了光强度的增加。
Negative phototactic orientation, step-up photophobic responses and light-induced action potentials have been studied in the ciliateStentor coeruleus. A resolved action spectrum, based on fluence rate-response curves, is consistent with stentorin as the photoreceptor. Calcium flux blockers prolong the response time for ciliary stop and reversal and inhibit step-up photophobic responses. Drugs believed to affect the membrane-bound calcium pump likewise inhibit phobic responses. On the other hand, α-phosphatidic acid promotes Ca2+-influx and enhances the photophobic sensitivity of the organism, thus providing an unambiguous evidence for the role of Ca2+influx. A change in the response time decreases the degree of phototactic orientation, indicating that negative phototaxis in this organism is brought about by subsequent phobic responses of individual rows of cilia as the associated photoreceptor granules experience an increase in light intensity when the organism rotates during forward locomotion in lateral light.