Enumeration of CD4+ T-Cells Using a Portable Microchip Count Platform in Tanzanian HIV-Infected Patients
Enumeration of CD4+ T-Cells Using a Portable Microchip Count Platform in Tanzanian HIV-Infected Patients
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DOI：
10.1371/journal.pone.0021409
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发表时间：
2011-07-06
期刊：
影响因子：
3.7
通讯作者：
Demirci, Utkan
中科院分区：
文献类型：
--
作者：
Moon, SangJun;Gurkan, Umut Atakan;Demirci, Utkan
背景：CD4（+）T淋巴细胞计数（CD4计数）是抗逆转录病毒治疗（ART）期间用于监测HIV感染者的标准方法。世界卫生组织 (WHO) 指出或建议，在资源匮乏的环境中，迫切需要一个手持式、即时护理、可靠且负担得起的 CD4 计数平台。 方法：使用我们开发的便携式、无标签微芯片 CD4 计数平台在即时检测 HIV 感染者的血液样本。总共收集了 130 份 HIV 感染者样本，其中包括来自布莱根妇女医院 (BWH) 的 16 份未识别的剩余血液样本，以及坦桑尼亚达累斯萨拉姆市艾滋病毒和艾滋病护理和治疗中心登记的穆希姆比利健康与联合科学大学 (MUHAS) 的 114 份剩余样本。对来自 BWH 和 MUHAS 的两个数据组进行了分析，并与普遍接受的 CD4 计数参考方法（FACSCalibur 系统）进行比较。结果：我们实验室（BWH）开发的便携式、电池供电、无显微镜微芯片平台（BWH）在 BWH（r = 0.94，p < 0.01）和 MUHAS（r = 0.49，p < 0.01）方面与 FACSCalibur 系统相比，显示 CD4 计数显着相关。这得到了 Bland-Altman 方法比较分析的支持。该设备使用内部开发的自动细胞计数程序在 10 分钟内快速产生 CD4 计数。结论：我们使用价格低廉的便携式平台获得了 HIV 感染者的 CD4 计数（
Background: CD4(+) T-lymphocyte count (CD4 count) is a standard method used to monitor HIV-infected patients during anti-retroviral therapy (ART). The World Health Organization (WHO) has pointed out or recommended that a handheld, point-of-care, reliable, and affordable CD4 count platform is urgently needed in resource-scarce settings.Methods: HIV-infected patient blood samples were tested at the point-of-care using a portable and label-free microchip CD4 count platform that we have developed. A total of 130 HIV-infected patient samples were collected that included 16 de-identified left over blood samples from Brigham and Women's Hospital (BWH), and 114 left over samples from Muhimbili University of Health and Allied Sciences (MUHAS) enrolled in the HIV and AIDS care and treatment centers in the City of Dar es Salaam, Tanzania. The two data groups from BWH and MUHAS were analyzed and compared to the commonly accepted CD4 count reference method (FACSCalibur system).Results: The portable, battery operated and microscope-free microchip platform developed in our laboratory (BWH) showed significant correlation in CD4 counts compared with FACSCalibur system both at BWH (r = 0.94, p < 0.01) and MUHAS (r = 0.49, p < 0.01), which was supported by the Bland-Altman methods comparison analysis. The device rapidly produced CD4 count within 10 minutes using an in-house developed automated cell counting program.Conclusions: We obtained CD4 counts of HIV-infected patients using a portable platform which is an inexpensive (