In vivo magnetic resonance imaging tracking of SPIO-labeled human umbilical cord mesenchymal stem cells
In vivo magnetic resonance imaging tracking of SPIO-labeled human umbilical cord mesenchymal stem cells
复制标题
SPIO标记的人脐带间充质干细胞的体内磁共振成像追踪
DOI：
10.1002/jcb.23432
复制
发表时间：
2012-03-01
影响因子：
4
通讯作者：
Feng, Hua
中科院分区：
文献类型：
--
作者：
Hu, Sheng-Li;Lu, Pei-Gang;Feng, Hua
超顺磁性氧化铁(SPIO)纳米粒子可以有效地标记人脐带间充质干细胞(Huc-MSCs)，在体外磁共振成像(MRI)上产生低信号。本研究旨在探讨应用无创性MRI技术对SPIO标记的人骨髓间充质干细胞进行体内示踪的可行性。分别加入浓度为0、7、14、28和56mgFe/ml(用DMEM/F12稀释)的培养液，孵育16h，普鲁士蓝染色检测标记效率。将大鼠随机分为对照组、Huc-MSCs组和SPIO标记的Huc-MSCs组。各组大鼠采用减重装置造成脊髓损伤。对大鼠进行神经功能检查。体内MRI示踪SPIO标记的Huc-MSCs移植到大鼠脊髓内。免疫荧光法检测Huc-MSCs的存活和迁移情况。在Huc-MSCs组中观察到明显的运动能力改善。与对照组比较，差异有统计学意义。体内MRI显示，注射SPIO标记的Huc-MSCs后1周和3周，移植区域的信号强度明显降低。未标记的Huc-MSCs的图像显示信号强度降低较小。移植的Huc-MSCs植入损伤大鼠脊髓内，存活至少8周。综上所述，Huc-MSCs移植后可在宿主脊髓内存活和迁移，促进脊髓损伤后的功能恢复。移植的SPIO标记的Huc-MSCs的无创性成像是可行的。J.细胞。生物化学。2012年，113：10051012。(C)2011年威利期刊公司。
Human umbilical cord mesenchymal stem cells (hUC-MSCs) can be efficiently labeled by superparamagnetic iron oxide (SPIO) nanoparticles, which produces low signal intensity on magnetic resonance imaging (MRI) in vitro. This study was to evaluate the feasibility of in vivo tracking for hUC-MSCs labeled by SPIO with noninvasive MRI. SPIO was added to cultures at concentrations equivalent to 0, 7, 14, 28, and 56 mu g Fe/ml (diluted with DMEM/F12) and incubated for 16?h. Prussian Blue staining was used to determinate the labeling efficiency. Rats were randomly divided into three groups, control group, hUC-MSCs group, and SPIO-labeled hUC-MSCs group. All groups were subjected to spinal cord injury (SCI) by weight drop device. Rats were examined for neurological function. In vivo MRI was used to track SPIO-labeled hUC-MSCs transplanted in rats spinal cord. Survival and migration of hUC-MSCs were also explored using immunofluorescence. Significant improvements in locomotion were observed in the hUC-MSCs groups. There was statistical significance compared with control group. In vivo MRI 1 and 3 weeks after injection showed a large reduction in signal intensity in the region transplanted with SPIO-labeled hUC-MSCs. The images from unlabeled hUC-MSCs showed a smaller reduction in signal intensity. Transplanted hUC-MSCs engrafted within the injured rats spinal cord and survived for at least 8 weeks. In conclusion, hUC-MSCs can survive and migrate in the host spinal cord after transplantation, which promote functional recovery after SCI. Noninvasive imaging of transplanted SPIO-labeled hUC-MSCs is feasible. J. Cell. Biochem. 113: 10051012, 2012. (C) 2011 Wiley Periodicals, Inc.