Direct stimulation by thyrotropin-releasing hormone (TRH) of polyphosphoinositide hydrolysis in GH3 cell membranes by a guanine nucleotide-modulated mechanism.
Direct stimulation by thyrotropin-releasing hormone (TRH) of polyphosphoinositide hydrolysis in GH3 cell membranes by a guanine nucleotide-modulated mechanism.
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促甲状腺激素释放激素 (TRH) 通过鸟嘌呤核苷酸调节机制直接刺激 GH3 细胞膜中的多磷酸肌醇水解。
DOI：
10.1016/0006-291x(85)91192-1
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发表时间：
1985
影响因子：
3.1
通讯作者：
Martin,TF
中科院分区：
文献类型：
--
作者：
Lucas,DO;Bajjalieh,SM;Kowalchyk,JA;Martin,TF
在促甲状腺素释放激素（TRH）反应性GH 3垂体细胞膜中检测了多磷酸肌醇水解。孵育[3 H]肌醇标记细胞的膜后产生[3 H]肌醇磷酸（IP 2和IP 3），表明存在膜相关聚磷酸肌醇磷酸二酯酶（PPI PDE）。TRH和GTP-γ-S以Ca 2+调节的方式刺激细胞膜PPI PDE活性。此外，TRH刺激的PPI水解被GTP增强。这些结果表明，直接在体外影响的激素PPI营业额，并建议参与的GTP结合成分在跨膜信号由TRH。
Polyphosphoinositide hydrolysis was examined in membranes from thyrotropin-releasing hormone (TRH)-responsive GH 3 pituitary cells.[3 H] Inositol phosphates (IP 2 and IP 3) were generated upon incubation of membranes from [3 H] inositol-labeled cells indicating the presence of a membrane-associated polyphosphoinositide phosphodiesterase (PPI PDE). Membrane PPI PDE activity was found to be stimulated by TRH and by GTP-γ-S in Ca 2+-modulated manner. In addition, TRH-stimulated PPI hydrolysis was potentiated by GTP. These results demonstrate direct in vitro effects of a hormone on PPI turnover and suggest the involvement of a GTP-binding component in transmembrane signalling by TRH.