OsDMC1 is required for homologous pairing in Oryza sativa
OsDMC1 is required for homologous pairing in Oryza sativa
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DOI：
10.1007/s11103-007-9195-2
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发表时间：
2007-09-01
影响因子：
5.1
通讯作者：
Wang, Tai
中科院分区：
文献类型：
--
作者：
Deng, Zhu-Yun;Wang, Tai
OsDMC 1是酵母DMC 1基因的水稻同源物。本研究利用RNA干扰技术分析了OsDMC 1在减数分裂中的功能。OsDMC 1-RNAi株系在营养生长期生长正常，但小穗和花粉不育。不育表型与RNAi介导的OsDMC 1转录和蛋白水平下调有关。进一步的细胞学观察显示，OsDMC 1基因敲除后，二价体形成缺陷，染色体分离不均等，孢子产生不规则，导致雄性减数分裂进程发生变化。荧光原位杂交结果显示OsDMC 1-RNAi株系存在同源配对缺陷。这些数据表明OsDMC 1是水稻减数分裂所必需的，并在同源配对中起重要作用。
OsDMC1 is the rice homologue of the yeast DMC1 gene. Here, we analyzed the function of OsDMC1 in meiosis using an RNA interference approach. The OsDMC1-RNAi lines grew normally during their vegetative phase but showed spikelet and pollen sterility. The sterility phenotypes were associated with down-regulated OsDMC1 transcript and protein levels mediated by RNAi. Further cytological observations of male meiocytes revealed that knock-down of OsDMC1 led to defects in bivalent formation and subsequent unequal chromosome segregation and irregular spore generation, and induced changes in male meiotic progression. Fluorescent in situ hybridization experiments revealed that the OsDMC1-RNAi lines were defective in homologous pairing. These data indicate that OsDMC1 is essential for rice meiosis and plays an important role in homologous pairing.