Tethering of ferredoxin:NADP plus oxidoreductase to thylakoid membranes is mediated by novel chloroplast protein TROL
Tethering of ferredoxin:NADP plus oxidoreductase to thylakoid membranes is mediated by novel chloroplast protein TROL
复制标题
DOI：
10.1111/j.1365-313x.2009.03999.x
复制
发表时间：
2009-12-01
期刊：
影响因子：
7.2
通讯作者：
Fulgosi, Hrvoje
中科院分区：
文献类型：
--
作者：
Juric, Snjezana;Hazler-Pilepic, Kroata;Fulgosi, Hrvoje
叶绿体类囊体膜中的两个光系统协同工作，产生从H2O到NADP+的线性电子流。铁氧还蛋白到NADP+的最终电子转移是通过黄素酶铁氧还蛋白：NADP+氧化还原酶(FNR)完成的。在这里，我们描述了TROL(类囊体罗丹斯蛋白)，它是类囊体膜上的一种核编码成分，在维管植物中连接FNR和维持有效的线性电子流(LEF)是必需的。Trol由两个不同的模块组成；一个位于中心位置的类罗丹尼斯结构域和一个C-末端疏水的FNR结合区。对拟南芥突变株系的分析表明，在缺乏TROL的情况下，高光下的相对电子传递速率严重降低，同时非光化学猝灭(NPQ)显著增加。因此，TROL可能代表了FNR、铁还蛋白和NADP+之间的复合体缺失的类囊体膜对接位点。这种结合可能是维持光合作用氧化还原平衡和提高NPQ所必需的。
P>Working in tandem, two photosystems in the chloroplast thylakoid membranes produce a linear electron flow from H2O to NADP+. Final electron transfer from ferredoxin to NADP+ is accomplished by a flavoenzyme ferredoxin:NADP+ oxidoreductase (FNR). Here we describe TROL (thylakoid rhodanese-like protein), a nuclear-encoded component of thylakoid membranes that is required for tethering of FNR and sustaining efficient linear electron flow (LEF) in vascular plants. TROL consists of two distinct modules; a centrally positioned rhodanese-like domain and a C-terminal hydrophobic FNR binding region. Analysis of Arabidopsis mutant lines indicates that, in the absence of TROL, relative electron transport rates at high-light intensities are severely lowered accompanied with significant increase in non-photochemical quenching (NPQ). Thus, TROL might represent a missing thylakoid membrane docking site for a complex between FNR, ferredoxin and NADP+. Such association might be necessary for maintaining photosynthetic redox poise and enhancement of the NPQ.