Rapid species identification of cooked poisonous mushrooms by using real-time PCR

Rapid species identification of cooked poisonous mushrooms by using real-time PCR
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DOI:
10.1128/aem.02082-07
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发表时间:
2008-05-01
影响因子:
4.4
通讯作者:
Aimi, Tadanori
Aimi, Tadanori
中科院分区:
生物学2区
文献类型:
--
作者:
Maeta, Kazuhiko;Ochi, Tomoya;Aimi, Tadanori

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利用实时荧光PCR技术对日本主要的熟毒蘑菇和鲜毒蘑菇进行了种属特异性鉴定。检测到特异性荧光信号,未检测到非特异性信号。因此,我们成功地开发了一种在1.5小时内鉴定有毒蘑菇的种特异性试验。
Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h.