Host microbiota modulates development of social preference in mice.
Host microbiota modulates development of social preference in mice.
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宿主微生物群调节小鼠社会偏好的发展。
DOI：
10.3402/mehd.v26.29719
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发表时间：
2015
影响因子：
--
通讯作者：
Diaz Heijtz R
中科院分区：
文献类型：
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作者：
Arentsen T;Raith H;Qian Y;Forssberg H;Diaz Heijtz R
越来越多的证据表明，本土肠道微生物群对大脑功能和行为具有持久的编程影响。在这项研究中，我们使用无菌（GF）小鼠模型，在整个发育过程中没有任何微生物群，以评估本土微生物群对社会偏好和重复行为（例如自我修饰）的影响。使用三室社交方法任务，我们证明，当成年 GF 小鼠选择与新的小鼠或物体共度时光时，与传统饲养的小鼠（无特定病原体，SPF）相比，它们花更多的时间嗅探和与刺激小鼠互动。然而，GF 和 SPF 小鼠在重复自我梳理行为上花费的时间没有差异。对杏仁核（社交脑网络一部分的关键区域）进行基于实时 PCR 的基因表达分析显示，与 SPF 小鼠相比，GF 小鼠的总脑源性神经营养因子 (BDNF)、含有 BDNF 外显子 I、IV、VI、IX 的转录物以及 NGFI-A（BDNF 下游信号分子）的 mRNA 水平显着降低。这些结果表明，本地微生物对杏仁核中 BDNF 外显子转录本的差异调节可能导致 GF 小鼠社会性发展的改变。
Mounting evidence indicates that the indigenous gut microbiota exerts long-lasting programming effects on brain function and behaviour. In this study, we used the germ-free (GF) mouse model, devoid of any microbiota throughout development, to assess the influence of the indigenous microbiota on social preference and repetitive behaviours (e.g. self-grooming). Using the three-chambered social approach task, we demonstrate that when adult GF mice were given a choice to spend time with a novel mouse or object, they spent significantly more time sniffing and interacting with the stimulus mouse compared to conventionally raised mice (specific pathogen-free, SPF). Time spent in repetitive self-grooming behaviour, however, did not differ between GF and SPF mice. Real-time PCR–based gene expression analysis of the amygdala, a key region that is part of the social brain network, revealed a significant reduction in the mRNA levels of total brain-derived neurotrophic factor (BDNF), BDNF exon I-, IV-, VI-, IX-containing transcripts, and NGFI-A (a signalling molecule downstream of BDNF) in GF mice compared to SPF mice. These results suggest that differential regulation of BDNF exon transcripts in the amygdala by the indigenous microbes may contribute to the altered social development of GF mice.