A novel optimised pre-embedding antibody-labelling correlative light electron microscopy technique
A novel optimised pre-embedding antibody-labelling correlative light electron microscopy technique
复制标题
一种新型优化的预嵌入抗体标记相关光电子显微镜技术
DOI：
10.1099/acmi.0.000750.v2
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Doyle N
中科院分区：
文献类型：
--
作者：
Doyle N
在复杂的细胞环境中，许多研究试图发现特定事件或感兴趣的物体的位置。近年来，显微镜技术的进步使得使用相关光电子显微镜（CLEM）可以对细胞的特定区域进行详细的观察。虽然这种强大的技术允许在共聚焦显微镜上的特定荧光区域与电子显微镜中的相同区域相关联，但它最常用于研究感兴趣的标记蛋白质。该方法适用于抗体标记的相关方法。我们已经证明，一些细胞结构对这一过程比其他细胞结构更敏感，这对于没有标记蛋白质或病毒或专用CLEM仪器的实验室来说是一项有用的技术。
In the intricate environment of a cell, many studies seek to discover the location of specific events or objects of interest. Advances in microscopy in recent years have allowed for high detail views of specific areas of cells of interest using correlative light electron microscopy (CLEM). While this powerful technique allows for the correlation of a specific area of fluorescence on a confocal microscope with that same area in an electron microscope, it is most often used to study tagged proteins of interest. This method adapts the correlative method for use with antibody labelling. We have shown that some cellular structures are more sensitive than others to this process and that this can be a useful technique for laboratories where tagged proteins or viruses, or dedicated CLEM instruments are not available.