Little samplers, big fleet: eDNA metabarcoding from commercial trawlers enhances ocean monitoring
Little samplers, big fleet: eDNA metabarcoding from commercial trawlers enhances ocean monitoring
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DOI：
10.1016/j.fishres.2022.106259
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发表时间：
2022-05
影响因子：
2.4
通讯作者：
Giulia Maiello;L. Talarico;P. Carpentieri;F. De Angelis;Simone Franceschini;Lynsey R. Harper;Erika F. Neave;O. Rickards;A. Sbrana;Peter Shum;Virginia Veltre;S. Mariani;T. Russo
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作者：
Giulia Maiello;L. Talarico;P. Carpentieri;F. De Angelis;Simone Franceschini;Lynsey R. Harper;Erika F. Neave;O. Rickards;A. Sbrana;Peter Shum;Virginia Veltre;S. Mariani;T. Russo
全球需要监测海洋资源状况，这是海洋管理的一项优先任务，但大多数海洋调查仍然依赖于昂贵且耗时的捕获技术。在这里，我们测试了一种新颖、易于使用的设备，用于在正常捕捞作业期间快速、轻松地在底拖网渔船上收集 eDNA：将定制的纱布卷绑在放置在渔网内的空心穿孔球形探针（“后探针”）上，旨在从周围环境中收集遗传物质的痕迹。我们从第勒尼安中部的三个遗址收集了六个样本。使用已建立的鱼类特定元条形码标记，我们恢复了超过 70% 的捕获物种，并准确地重建了所考虑的不同测深层的典型鱼类组合。 eDNA 元条形码数据还返回了大部分中层物种的生物多样性“奖励”，这些物种无法通过底拖网捕获。需要进一步的研究来升级这种有前途的方法，使其成为监测渔获物构成、评估种群分布以及总体记录整个海洋鱼类群落变化的有力工具。
The global need to monitor the status of marine resources is a priority task in marine management, but most ocean surveys still rely on costly and time-consuming capture-based techniques. Here we test a novel, easy-to use device to collect eDNA on board of bottom trawl fishing vessels, during normal fishing operations, quickly and easily: custom-made rolls of gauze tied to a hollow perforated spherical probe (the ‘metaprobe’) that placed inside the fishing net aims to gather traces of genetic material from the surrounding environment. We collected six samples from three central Tyrrhenian sites. Using an established fish-specific metabarcoding marker, we recovered over 70% of the caught species and accurately reconstructed fish assemblages typical of the different bathymetric layers considered. eDNA metabarcoding data also returned a biodiversity ‘bonus’ of mostly mesopelagic species, not catchable by bottom trawls. Further investigation is needed to upscale this promising approach as a powerful tool to monitor catch composition, assess the distribution of stocks, and generally record changes in fish communities across the oceans.