Induction of two genes by glucose starvation in hamster fibroblasts.
Induction of two genes by glucose starvation in hamster fibroblasts.
复制标题
仓鼠成纤维细胞中葡萄糖饥饿诱导两个基因。
DOI：
10.1073/pnas.81.4.988
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发表时间：
1984
影响因子：
11.1
通讯作者：
Lee,AS
中科院分区：
文献类型：
--
作者：
Lin,AY;Lee,AS
在仓鼠成纤维细胞系K12中证明了葡萄糖饥饿特异性诱导的两个基因的协调表达。使用两个cDNA质粒，p4A3和p3C5，作为杂交探针，我们研究这些基因的诱导动力学时，细胞生长在培养基中剥夺葡萄糖。结果表明，（i）在约8小时的滞后期后，p4A3和p3C5 mRNA水平快速且同时增加，（ii）p4A3和p3C5 mRNA水平的升高主要是由于新的转录。此外，我们比较了这些葡萄糖调节基因在培养细胞和磷酸烯醇式丙酮酸羧激酶，在禁食大鼠中的代谢的限速步骤催化的mRNA转录。我们的研究结果表明，磷酸烯醇式丙酮酸羧激酶的表达是不能诱导的葡萄糖饥饿在我们的培养细胞。
The coordinated expression of two genes specifically induced by glucose starvation is demonstrated in a hamster fibroblast cell line, K12. Using two cDNA plasmids, p4A3 and p3C5, as hybridization probes, we examine the kinetics of induction of these genes when the cells are grown in medium deprived of glucose. The results show that (i) after a lag period of about 8 hr, there is a rapid and simultaneous increase of the p4A3 and p3C5 mRNA levels and (ii) the elevation of the mRNA levels for p4A3 and p3C5 is largely due to new transcription. In addition, we compare the mRNA transcripts encoded by these glucose-regulated genes in culture cells and phosphoenolpyruvate carboxykinase, the enzyme that catalyzes the rate-limiting step in gluconeogenesis in fasted rats. Our results indicate that the expression of phosphoenolpyruvate carboxykinase is not inducible by glucose starvation in our culture cells.